Li WW, Yuan H, Kong S, Tian SB. E3 ubiquitin ligase TRIM55 promotes metastasis of gastric cancer cells by mediating epithelial-mesenchymal transition. World J Gastrointest Oncol 2022; 14(11): 2183-2194 [PMID: 36438697 DOI: 10.4251/wjgo.v14.i11.2183]
Corresponding Author of This Article
Shu-Bo Tian, PhD, Chief Doctor, Surgeon, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No. 324 Jingwuweiqi Road, Jinan 250021, Shandong Province, China. ttkl_bo@126.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastrointest Oncol. Nov 15, 2022; 14(11): 2183-2194 Published online Nov 15, 2022. doi: 10.4251/wjgo.v14.i11.2183
E3 ubiquitin ligase TRIM55 promotes metastasis of gastric cancer cells by mediating epithelial-mesenchymal transition
Wei-Wei Li, Hao Yuan, Shuai Kong, Shu-Bo Tian
Wei-Wei Li, Department of Pulmonary and Critical Care Medicine, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, Shandong Province, China
Wei-Wei Li, Department of Critical Care Medicine, The 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250031, Shandong Province, China
Hao Yuan, Shuai Kong, Shu-Bo Tian, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong Province, China
Shuai Kong, Shu-Bo Tian, Department of Gastrointestinal Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, Shandong Province, China
Author contributions: Li WW and Yuan H contributed equally to this work; Tian SB and Li WW designed the study; Li WW and Yuan H performed all experiments; Kong S and Tian SB analyzed the data and drafted the article; All authors read and approved the final manuscript.
Supported byShandong Province Medicine and Health Science and Technology Development Plan Project, No. 2019WS477.
Institutional review board statement: The study was reviewed and approved by the Shandong Provincial Hospital Affiliated to Shandong First Medical University Institutional Review Board.
Institutional animal care and use committee statement: No animal subjects were involved in our experiments.
Informed consent statement: All study participants or their legal guardian provided informed written consent about personal and medical data collection prior to study enrolment.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at ttkl_bo@126com.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Shu-Bo Tian, PhD, Chief Doctor, Surgeon, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No. 324 Jingwuweiqi Road, Jinan 250021, Shandong Province, China. ttkl_bo@126.com
Received: August 21, 2022 Peer-review started: August 21, 2022 First decision: September 13, 2022 Revised: September 28, 2022 Accepted: October 27, 2022 Article in press: October 27, 2022 Published online: November 15, 2022 Processing time: 85 Days and 19.2 Hours
ARTICLE HIGHLIGHTS
Research background
TRIM55 plays important role in hepatocellular carcinoma and lung adenocarcinoma. However, little is known about the role of TRIM55 in gastric cancer (GC).
Research motivation
To discover the targets for the diagnosis, treatment and prognosis prediction of GC.
Research objectives
To explore the biological function of TRIM55 and its underlying molecular mechanism in GC.
Research methods
The expression of TRIM55 was determined by quantitative real-time polymerase chain reaction and Western blot. Cell counting kit-8 assay, colony formation, wound healing assay and transwell assay were used to investigate the TRIM55 function.
Research results
TRIM55 expression levels were significantly increased in GC cell lines and tissues. High expression of TRIM55 was correlated with poor prognosis of GC patients. Knockdown of TRIM55 in GC cell lines inhibited proliferation, colony formation, migration and invasion in vitro. TRIM55 can regulate the expression of epithelial-mesenchymal transition-related proteins in GC cells.
Research conclusions
TRIM55 functions as an oncogene through promoting cell proliferation, migration and invasion in GC.
Research perspectives
TRIM55 may be a new potential target in GC treatment.
