Li WW, Yuan H, Kong S, Tian SB. E3 ubiquitin ligase TRIM55 promotes metastasis of gastric cancer cells by mediating epithelial-mesenchymal transition. World J Gastrointest Oncol 2022; 14(11): 2183-2194 [PMID: 36438697 DOI: 10.4251/wjgo.v14.i11.2183]
Corresponding Author of This Article
Shu-Bo Tian, PhD, Chief Doctor, Surgeon, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No. 324 Jingwuweiqi Road, Jinan 250021, Shandong Province, China. ttkl_bo@126.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastrointest Oncol. Nov 15, 2022; 14(11): 2183-2194 Published online Nov 15, 2022. doi: 10.4251/wjgo.v14.i11.2183
E3 ubiquitin ligase TRIM55 promotes metastasis of gastric cancer cells by mediating epithelial-mesenchymal transition
Wei-Wei Li, Hao Yuan, Shuai Kong, Shu-Bo Tian
Wei-Wei Li, Department of Pulmonary and Critical Care Medicine, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, Shandong Province, China
Wei-Wei Li, Department of Critical Care Medicine, The 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250031, Shandong Province, China
Hao Yuan, Shuai Kong, Shu-Bo Tian, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong Province, China
Shuai Kong, Shu-Bo Tian, Department of Gastrointestinal Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, Shandong Province, China
Author contributions: Li WW and Yuan H contributed equally to this work; Tian SB and Li WW designed the study; Li WW and Yuan H performed all experiments; Kong S and Tian SB analyzed the data and drafted the article; All authors read and approved the final manuscript.
Supported byShandong Province Medicine and Health Science and Technology Development Plan Project, No. 2019WS477.
Institutional review board statement: The study was reviewed and approved by the Shandong Provincial Hospital Affiliated to Shandong First Medical University Institutional Review Board.
Institutional animal care and use committee statement: No animal subjects were involved in our experiments.
Informed consent statement: All study participants or their legal guardian provided informed written consent about personal and medical data collection prior to study enrolment.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at ttkl_bo@126com.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Shu-Bo Tian, PhD, Chief Doctor, Surgeon, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No. 324 Jingwuweiqi Road, Jinan 250021, Shandong Province, China. ttkl_bo@126.com
Received: August 21, 2022 Peer-review started: August 21, 2022 First decision: September 13, 2022 Revised: September 28, 2022 Accepted: October 27, 2022 Article in press: October 27, 2022 Published online: November 15, 2022 Processing time: 85 Days and 19.2 Hours
Abstract
BACKGROUND
Gastric cancer (GC) is considered a major global health problem. The role of TRIM55, a member of the three-domain protein family, in GC is unknown.
AIM
To determine the expression of TRIM55 in GC tissues and its relationship with clinicopathological characteristics, and to investigate the effects of TRIM55 on the malignant biological behavior of GC cells.
METHODS
Differential expression of TRIM55 in GC and para-cancer tissues was detected by immunohistochemistry, and the relationship between TRIM55 level and clinicopathological characteristics and prognosis was analyzed. Gain-of-function, loss-of-function, cell counting kit-8 assay, colony formation, transwell assay, wound healing assay, and western blot analysis were used to assess the potential role of TRIM55 in the development of GC.
RESULTS
TRIM55 expression was significantly increased in GC tissues compared with adjacent normal tissues. High expression of TRIM55 was associated with advanced pathological stage and poor prognosis. Overexpression of TRIM55 promoted invasion and metastasis of GC cells in vitro by regulating epithelial-mesenchymal transition (EMT), whereas knockdown of TRIM55 had the opposite effect. Our data showed that TRIM55 is highly expressed in GC tissues, and is associated with poor prognosis. TRIM55 plays the role of an oncogene in GC, and it promotes metastasis of GC through the regulation of EMT.
CONCLUSION
TRIM55 may be a possible target for the diagnosis and prognosis of GC patients.
Core Tip: TRIM55 expression was elevated in gastric cancer (GC) cancer tissues. Depletion of TRIM55 in GC cells suppressed proliferation, migration and invasion of cells. Knockdown of TRIM55 affected the expression of cell epithelial-mesenchymal transition-related proteins. TRIM55 may serve as an oncogene in GC.
