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©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
Mucin 1 and interleukin-11 protein expression and inflammatory reactions in the intestinal mucosa of necrotizing enterocolitis children after surgery
Hong-Xia Pan, Chang-Song Zhang, Chia-Hui Lin, Min-Min Chen, Xiao-Zhong Zhang, Nong Yu
Hong-Xia Pan, Min-Min Chen, Department of Clinical Laboratory Medicine, Suzhou BenQ Medical Center, Affiliated BenQ Hospital of Nanjing Medical University, Suzhou 215011, Jiangsu Province, China
Chang-Song Zhang, Department of Clinical Laboratory Medicine, The Affiliated Suzhou Science and Technology Town Hospital, Nanjing Medical University, Suzhou 215153, Jiangsu Province, China
Chia-Hui Lin, General Manager's Office, Suzhou Gallant Biotech Biotechnology Co. Ltd, Suzhou 215163, Jiangsu Province, China
Xiao-Zhong Zhang, Department of Cardiology, National High Tech Development Zone Hospital, Suzhou 215129, Jiangsu Province, China
Nong Yu, Department of Laboratory Medicine, National High Tech Development Zone Hospital, Suzhou 215129, Jiangsu Province, China
Author contributions: Pan HX and Zhang CS have the same contribution to this article and should be regarded as co-first authors; Pan HX and Zhang CS design the experiment; Lin CH drafted the work, Chen MM, Zhang XZ and Yu N collected the data; Pan HX and Zhang CS interpreted data; Pan HX, Zhang CS and Yu N wrote the article.
Supported by Suzhou Science and Technology Program, No. SLT202005; Suzhou Municipal Commission of Health and Family Planning, No. LCZX202031; Suzhou New District Science and Technology Plan, No. 2019Z009; and Independent Innovation Project of National High Tech Development Zone Hospital, No. SGY2018C03.
Institutional review board statement: This study was approved by the Affiliated BenQ Hospital of Nanjing Medical University Ethics Committee.
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: No conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
http://creativecommons.org/Licenses/by-nc/4.0/ Corresponding author: Nong Yu, BM BCh, Chief Technician, Department of Laboratory Medicine, National High Tech Development Zone Hospital, No. 95 Huashan Road, Huqiu District, Suzhou 215129, Jiangsu Province, China. yunong78612@sina.com
Received: April 22, 2021
Peer-review started: April 22, 2021
First decision: May 24, 2021
Revised: June 28, 2021
Accepted: July 28, 2021
Article in press: July 28, 2021
Published online: September 6, 2021
ARTICLE HIGHLIGHTS
Research background
In the clinic, neonatal necrotizing enterocolitis (NEC) is a frequently occurring pediatric intestinal inflammatory disease. The mortality of premature infants with NEC is as high as 50%, and the incidence shows an increasing trend, which has serious impacts on the quality of life of children.
Research motivation
This study provides help for the clinical diagnosis and treatment of neonatal NEC.
Research objectives
This study aimed to investigate the expression and significance of mucin 1 (MUC1) and interleukin-11 (IL-11) in the intestinal mucosa of children with neonatal NEC after surgery.
Research methods
Total 48 postoperative intestinal mucosal specimens from children with NEC (NEC group) and 22 intestinal mucosal specimens from congenital intestinal atresia (control group) were collected in our hospital. Immunohistochemical staining and Western-blot technique were used to detect the expression of MUC-1 protein and IL-11 protein in the two groups. The serum levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the two groups were detected by enzyme-linked immunosorbent assay, and the relationship between MUC-1 protein, IL-11 protein and serum TNF-α and IL-1β was analyzed by linear correlation method.
Research results
The protein expression of MUC-1 and IL-11 in the NEC group was significantly lower than that in the control group, and the difference was statistically significant. The levels of serum TNF-α and IL-1β in the NEC group were significantly higher than those in the control group. The protein expression of MUC-1 and IL-11 in the NEC group negatively correlated with serum TNF-α and IL-1β levels. There was a significant negative correlation between the protein expression of MUC-1 and IL-11 and the levels of serum TNF-α and IL-1β in the NEC group.
Research conclusions
The protein expression of MUC1 and IL-11 in the intestinal mucosa of children with NEC is significantly downregulated after surgery. This down regulation may be involved in the pathogenesis of the disease and has a certain correlation with inflammatory response factors in children with NEC.
Research perspectives
MUC1 and IL-11 proteins may be related to the pathogenesis of the disease and have a certain correlation with inflammatory response factors in children with NEC, which has profound clinical significance.
