Pan HX, Zhang CS, Lin CH, Chen MM, Zhang XZ, Yu N. Mucin 1 and interleukin-11 protein expression and inflammatory reactions in the intestinal mucosa of necrotizing enterocolitis children after surgery. World J Clin Cases 2021; 9(25): 7372-7380 [PMID: 34616804 DOI: 10.12998/wjcc.v9.i25.7372]
Corresponding Author of This Article
Nong Yu, BM BCh, Chief Technician, Department of Laboratory Medicine, National High Tech Development Zone Hospital, No. 95 Huashan Road, Huqiu District, Suzhou 215129, Jiangsu Province, China. yunong78612@sina.com
Research Domain of This Article
Pediatrics
Article-Type of This Article
Retrospective Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Hong-Xia Pan, Min-Min Chen, Department of Clinical Laboratory Medicine, Suzhou BenQ Medical Center, Affiliated BenQ Hospital of Nanjing Medical University, Suzhou 215011, Jiangsu Province, China
Chang-Song Zhang, Department of Clinical Laboratory Medicine, The Affiliated Suzhou Science and Technology Town Hospital, Nanjing Medical University, Suzhou 215153, Jiangsu Province, China
Chia-Hui Lin, General Manager's Office, Suzhou Gallant Biotech Biotechnology Co. Ltd, Suzhou 215163, Jiangsu Province, China
Xiao-Zhong Zhang, Department of Cardiology, National High Tech Development Zone Hospital, Suzhou 215129, Jiangsu Province, China
Nong Yu, Department of Laboratory Medicine, National High Tech Development Zone Hospital, Suzhou 215129, Jiangsu Province, China
Author contributions: Pan HX and Zhang CS have the same contribution to this article and should be regarded as co-first authors; Pan HX and Zhang CS design the experiment; Lin CH drafted the work, Chen MM, Zhang XZ and Yu N collected the data; Pan HX and Zhang CS interpreted data; Pan HX, Zhang CS and Yu N wrote the article.
Supported bySuzhou Science and Technology Program, No. SLT202005; Suzhou Municipal Commission of Health and Family Planning, No. LCZX202031; Suzhou New District Science and Technology Plan, No. 2019Z009; and Independent Innovation Project of National High Tech Development Zone Hospital, No. SGY2018C03.
Institutional review board statement: This study was approved by the Affiliated BenQ Hospital of Nanjing Medical University Ethics Committee.
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: No conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Nong Yu, BM BCh, Chief Technician, Department of Laboratory Medicine, National High Tech Development Zone Hospital, No. 95 Huashan Road, Huqiu District, Suzhou 215129, Jiangsu Province, China. yunong78612@sina.com
Received: April 22, 2021 Peer-review started: April 22, 2021 First decision: May 24, 2021 Revised: June 28, 2021 Accepted: July 28, 2021 Article in press: July 28, 2021 Published online: September 6, 2021 Processing time: 130 Days and 22.1 Hours
Abstract
BACKGROUND
Necrotizing enterocolitis (NEC) of the newborn is a frequently occurring clinical disease in infants. The mortality rate of NEC in premature infants is as high as 50%, and the morbidity rate is on the rise. NEC has already caused serious impacts on newborn survival and poses serious threats to both children and families.
AIM
To investigate the expression and significance of mucin 1 (MUC1) and interleukin-11 (IL-11) in the intestinal mucosa of infants with neonatal NEC after surgery.
METHODS
Forty-eight postoperative intestinal mucosal specimens from children with NEC (NEC group) and twenty-two intestinal mucosal specimens from children with congenital intestinal atresia (control group) were collected in our hospital. Immunohistochemical staining and Western blot analysis were used to examine the protein expression of MUC-1 and IL-11 in the two groups. The serum levels of tumor necrosis factor-α (TNF-α) and IL-1β in the two groups were measured by enzyme-linked immunosorbent assay, and the relationship between MUC-1 and IL-11 protein expression and serum TNF-α and IL-1β levels was analyzed by the linear correlation method.
RESULTS
The protein expression of MUC-1 and IL-11 in the NEC group was significantly lower than that in the control group, and the difference was statistically significant (P < 0.05). The levels of serum TNF-α and IL-1β in the NEC group were significantly higher than those in the control group (P < 0.05). The protein expression of MUC-1 and IL-11 in the NEC group negatively correlated with serum TNF-α and IL-1β levels (P < 0.05). There was a significant negative correlation between the protein expression of MUC-1 and IL-11 and the levels of serum TNF-α and IL-1β in the NEC group.
CONCLUSION
The protein expression of MUC1 and IL-11 in the intestinal mucosa of children with NEC is significantly downregulated after surgery. This downregulation may be involved in the pathogenesis of this disease and has a certain correlation with inflammatory response factors in children with NEC.
Core Tip: This study analyzed the expression changes of mucin 1 and interleukin-11 in intestinal mucosa after necrotizing enterocolitis operation in neonatal necrotizing enterocolitis. It may be related to the pathogenesis of the disease, and providing clinical guidance and basis.
