Case Control Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Jun 16, 2022; 10(17): 5551-5565
Published online Jun 16, 2022. doi: 10.12998/wjcc.v10.i17.5551
Gut peptide changes in patients with obstructive jaundice undergoing biliary drainage: A prospective case control study
Tajana Pavić, Stipe Pelajić, Nina Blažević, Dominik Kralj, Milan Milošević, Ivana Mikolasevic, Ivan Lerotic, Davor Hrabar
Tajana Pavić, Stipe Pelajić, Nina Blažević, Dominik Kralj, Ivan Lerotic, Davor Hrabar, Department of Gastroenterology and Hepatology, University Hospital Center Sestre Milosrdnice, Zagreb 10000, Croatia
Milan Milošević, Andrija Stampar School of Public Health WHO Collaborative Centre for Occupational Health, University of Zagreb, School of Medicine, Zagreb 10000, Croatia
Ivana Mikolasevic, Department of Gastroenterology, University Hospital Center Rijeka, Rijeka 51000, Croatia
Author contributions: Pavić T contributed to study design and conception and collected the data; Pelajić S, Blažević N, Kralj D, and Mikolasevic I wrote the draft of the manuscript and contributed to data interpretation; Milošević M performed statistical analysis; Lerotic I collected the data; Hrabar D served as scientific advisor and guided the study.
Institutional review board statement: The study was reviewed and approved by the University Hospital Center Sestre Milosrdnice Ethics Committee, No. EP-15584/10.
Conflict-of-interest statement: The authors declare that there is no conflict of interest to disclose.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at tajana.pavic@gmail.com.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Tajana Pavić, MD, PhD, Assistant Professor, Department of Gastroenterology and Hepatology, University Hospital Center Sestre Milosrdnice, Vinogradska cesta 29, Zagreb 10000, Croatia. tajana.pavic@gmail.com
Received: December 10, 2021
Peer-review started: December 10, 2021
First decision: January 8, 2022
Revised: January 20, 2022
Accepted: April 9, 2022
Article in press: April 9, 2022
Published online: June 16, 2022
Processing time: 180 Days and 9.8 Hours
Abstract
BACKGROUND

Biliary obstruction is a relatively common condition that affects approximately 5 in 1000 people annually. Malnutrition is very common in patients with biliary obstruction and since it is associated with significant morbidity and mortality, it is important to identify factors and mechanisms involved in its development.

AIM

To determine the influence of obstructive jaundice on the hormones controlling appetite and nutritive status.

METHODS

This was a prospective case control study performed in a tertiary center in Zagreb, Croatia. Patients with biliary obstruction undergoing internal biliary drainage from September 2012 until August 2013 were enrolled. After excluding patients who developed procedure related complications or were lost in the follow-up, out of initial 73 patients, 55 patients were included in the analysis, including 34 with benign and 21 with malignant disease. Meanwhile, 40 non-jaundiced controls were also included. Appetite, nutritional status, and serum ghrelin, cholecystokinin (CCK), interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α) were determined at admission, 48 h and 28 d after internal biliary drainage. Chi square test was used for categorical variables. Continuous variables were analysed for normality by Kolmogorov–Smirnov test and relevant non-parametric (Mann-Whitney, Kruskal-Wallis, and Friedman) or parametric (t-test and analysis of variance) tests were used.

RESULTS

Patients with obstructive jaundice were significantly malnourished compared to controls, regardless of disease etiology. Plasma ghrelin and CCK levels were significantly higher in patients with obstructive jaundice. Serum bilirubin concentrations were negatively correlated with ghrelin levels and positively correlated with TNF-α, but had no correlation with CCK concentrations. After internal biliary drainage, a significant improvement of nutritional status was observed although serum concentrations of ghrelin, IL-6, and TNF-α remained significantly elevated even 28 d after the procedure. CCK levels in patients without malnutrition remained elevated 28 d after the procedure, but in patients with malnutrition, CCK levels decreased to levels comparable with those in the control group. We have not established any correlation between appetite and serum levels of ghrelin, CCK, IL-6, and TNF-α before and after biliary drainage.

CONCLUSION

Possible abnormalities in ghrelin and CCK regulation may be associated with the development of malnutrition during the inflammatory response in patients with biliary obstruction.

Keywords: Ghrelin; Cholecystokinin; Biliary obstruction; Malnutrition

Core Tip: Biliary obstruction is a common condition with subsequent malnutrition that contributes to patient’s high morbidity and mortality. Therefore, it is important to better understand the interaction of hormonal and inflammatory parameters on nutritional status. We conducted a case control study to determine the influence of obstructive jaundice on the hormones controlling appetite and nutritional status and showed that patients with obstructive jaundice had worse nutritional status compared to controls, regardless of disease etiology. Plasma ghrelin and cholecystokinin levels were significantly higher in patients with obstructive jaundice, which may be associated with the development of malnutrition during the inflammatory response.