Sun CB, Wang HY, Han XQ, Liu YN, Wang MC, Zhang HX, Gu YF, Leng XG. LINC00511 promotes gastric cancer cell growth by acting as a ceRNA. World J Gastrointest Oncol 2020; 12(4): 394-404 [PMID: 32368318 DOI: 10.4251/wjgo.v12.i4.394]
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Chong-Bing Sun, Yong-Ning Liu, Meng-Chun Wang, Department of General Surgery, Weifang People's Hospital, Weifang 261041, Shandong Province, China
Hong-Yi Wang, Hong-Xia Zhang, You-Feng Gu, Xiao-Gang Leng, Department of Anorectal Surgery, Weifang People's Hospital, Weifang 261041, Shandong Province, China
Xiao-Qing Han, Department of Spine Surgery, Weifang People's Hospital, Weifang 261041, Shandong Province, China
Author contributions: Sun CB, Wang HY, Han XQ, Liu YQ, and Wang MC analyzed and interpreted data and wrote the article; Zhang HX, Gu YF and Leng XG drafted the work and collected the data.
Institutional review board statement: Approved by the hospital ethics committee of Weifang People's Hospital.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Received: December 21, 2019 Peer-review started: December 21, 2019 First decision: January 19, 2020 Revised: February 4, 2020 Accepted: March 22, 2020 Article in press: March 22, 2020 Published online: April 15, 2020
ARTICLE HIGHLIGHTS
Research background
It has been reported that LINC00511 contributes to tumorigenesis in various diseases. However, the role of LINC00511 in gastric cancer (GC) cell growth remains mostly unknown.
Research motivation
The specific biological function and regulatory mechanism of LINC00511 in GC have not been extensively explored.
Research objectives
In this study, the authors aimed to determine whether the long noncoding RNAs LINC00511 exerted its carcinogenic function in GC via the miR-124-3p/PDK4 axis.
Research methods
The cell culture and transfection, RNA extraction and quantitative real-time PCR, CCK-8 assay, Colony formation assay, Luciferase reporter assay, RIP assay, RNA pull-down assay, and Western blot analysis were used to show expression and mechanisms of LINC00511 in GC progression and apoptosis. Rescue assays were performed to verify the relationships among LINC00511, miR-124-3p and PDK4 further.
Research results
The expression of LINC00511 was remarkably upregulated in GC cells compared to that in corresponding normal cell lines. Compared to the controls, cell proliferation was inhibited, and cell apoptosis was increased upon LINC00511 knockdown, demonstrating that LINC00511 influenced GC cell growth. An exploration of the molecular mechanism revealed that LINC00511 functioned as a molecular sponge of miR-124-3p and that PDK4 was a downstream target of miR-124-3p in GC.
Research conclusions
The results of the study demonstrate that LINC00511 promotes gastric cancer cell growth by acting as a ceRNA to regulate the miR-124-3p/PDK4 axis, which may be a promising therapeutic target for GC.
