Case Report
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Nov 16, 2021; 9(32): 9903-9910
Published online Nov 16, 2021. doi: 10.12998/wjcc.v9.i32.9903
Next-generation sequencing technology for diagnosis and efficacy evaluation of a patient with visceral leishmaniasis: A case report
Zhou-Ning Lin, Yong-Cheng Sun, Jia-Ping Wang, Yan-Li Lai, Li-Xia Sheng
Zhou-Ning Lin, Internal Medicine, School of Medicine Ningbo University, Ningbo 315000, Zhejiang Province, China
Yong-Cheng Sun, Jia-Ping Wang, Yan-Li Lai, Li-Xia Sheng, Department of Hematology, Ningbo First Hospital, Ningbo 315000, Zhejiang Province, China
Author contributions: Lin ZN reviewed the literature and contributed to the manuscript drafting; Sun YC, Wang JP and Lai YL collected the clinical data and reviewed the literature; Sheng LX reviewed the manuscript; all authors issued approval of the final version.
Supported by National Nature Science Foundation of China, No. 81401321; Basic Public Welfare Research Project of Zhejiang Province, No. LGF19H080002; Science Research Project of Medicine and Hygiene of Zhejiang Province, No. 2018PY052; Public Welfare Science and Technology Project of Ningbo, No. 2019C50068.
Informed consent statement: Informed consent was obtained from the patient.
Conflict-of-interest statement: The authors declare that they have no conflicts of interest.
CARE Checklist (2016) statement: The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Li-Xia Sheng, PhD, Chief Doctor, Department of Hematology, Ningbo First Hospital, No. 59, Liuting Street, Haishu District, Ningbo 315000, Zhejiang Province, China. slx800408@163.com
Received: June 6, 2021
Peer-review started: June 6, 2021
First decision: June 25, 2021
Revised: July 9, 2021
Accepted: September 15, 2021
Article in press: September 15, 2021
Published online: November 16, 2021
Processing time: 156 Days and 8.6 Hours
Core Tip

Core Tip: Visceral leishmaniasis (VL) is an easily overlooked parasitic disease because of its low incidence and atypical clinical manifestations. Here, we report a case of imported VL. Initially, no Leishmania was found in the bone marrow or by spleen biopsy. Leishmania was finally confirmed by metagenomic next-generation sequencing (mNGS) analysis of peripheral blood. Finally, after treatment with amphotericin B, the patient recovered well, and various indicators gradually returned to normal during the follow-up period. As a rapid and accurate detection method, mNGS can be used as an alternative method to diagnose and evaluate suspicious cases.