Retrospective Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Sep 16, 2021; 9(26): 7693-7703
Published online Sep 16, 2021. doi: 10.12998/wjcc.v9.i26.7693
Clinical metagenomic sequencing for rapid diagnosis of pneumonia and meningitis caused by Chlamydia psittaci
Xiao-Wei Yin, Zheng-Dao Mao, Qian Zhang, Qiu-Xiang Ou, Jia Liu, Yang Shao, Zhi-Guang Liu
Xiao-Wei Yin, Zheng-Dao Mao, Qian Zhang, Zhi-Guang Liu, Department of Respiratory and Critical Care Medicine, Changzhou Second People’s Hospital Affiliated to Nanjing Medical University, Changzhou 213164, Jiangsu Province, China
Qiu-Xiang Ou, Yang Shao, Research & Development, Nanjing Geneseeq Technology Inc., Nanjing 210032, Jiangsu Province, China
Jia Liu, Research & Development, Dinfectome Inc., Nanjing 213164, Jiangsu Province, China
Yang Shao, School of Public Health, Nanjing Medical University, Nanjing 213164, Jiangsu Province, China
Author contributions: Yin XW contributed to the study concept and design and drafting of the manuscript; Mao ZD and Zhang Q contributed to the acquisition of data; Ou QX and Liu J contributed to the interpretation of data and drafting of the manuscript; Shao Y contributed to critical revision of the manuscript; Liu ZG supervised the study; all authors have read and approved the final manuscript.
Institutional review board statement: This study was reviewed and approved by the Ethics Committee of Changzhou Second People’s Hospital affiliated to Nanjing Medical University, China (approval No. 2020KY268-01).
Informed consent statement: All study participants provided informed consent prior to sample collection according to the institutional guidelines and gave permissions to the use of their clinical data and accompanying images.
Conflict-of-interest statement: Ou QX and Shao Y are employees of Nanjing Geneseeq Technology Inc; Liu J is an employee of Dinfectome Inc. The remaining authors have nothing to disclose.
Data sharing statement: The authors confirm that the data supporting the findings of this study are available within the article.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Zhi-Guang Liu, MD, Doctor, Department of Respiratory and Critical Care Medicine, Changzhou Second People’s Hospital Affiliated to Nanjing Medical University, No. 68 Gehu Road, Wujin District, Changzhou 213164, Jiangsu Province, China. 545485079@qq.com
Received: February 18, 2021
Peer-review started: February 18, 2021
First decision: April 13, 2021
Revised: April 15, 2021
Accepted: July 2, 2021
Article in press: July 2, 2021
Published online: September 16, 2021
Processing time: 197 Days and 4.8 Hours
Core Tip

Core Tip: Detection of Chlamydia psittaci (C. psittaci) infections relies on traditional antigen-based immunoassays or serological testing that often lack sensitivity and/or specificity. Our data not only reinforce that metagenomic next generation sequencing represents a valuable tool for rapid, sensitive, and accurate pathogen detection, including the identification of C. psittaci, but also raise public health concerns over C. psittaci infections.