Case Report
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Jul 16, 2021; 9(20): 5621-5630
Published online Jul 16, 2021. doi: 10.12998/wjcc.v9.i20.5621
Rapid diagnosis of disseminated Mycobacterium mucogenicum infection in formalin-fixed, paraffin-embedded specimen using next-generation sequencing: A case report
Jing Liu, Zi-Ying Lei, Yi-Hua Pang, Ying-Xiong Huang, Le-Jia Xu, Jian-Yun Zhu, Jia-Xing Zheng, Xiao-Hua Yang, Bing-Liang Lin, Zhi-Liang Gao, Chao Zhuo
Jing Liu, Zi-Ying Lei, Yi-Hua Pang, Le-Jia Xu, Jian-Yun Zhu, Jia-Xing Zheng, Xiao-Hua Yang, Bing-Liang Lin, Zhi-Liang Gao, Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China
Ying-Xiong Huang, Department of Emergency, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510000, Guangdong Province, China
Le-Jia Xu, Department of Pharmacy, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China
Chao Zhuo, State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong Province, China
Author contributions: Liu J and Lei ZY contributed equally to this manuscript and should be regarded as co-first authors; Liu J and Lei ZY were the patient’s physicians, and collected and analyzed the patient’s medical data, wrote and revised the manuscript; Pang YH, Huang YX, Xu LJ, Zhu JY, Zheng JX, Yang XH and Lin BL participated in the treatment of the patient during hospitalization; Gao ZL and Zhuo C made a critical contribution to the treatment plan of the patient and critically revised the manuscript for important intellectual content, they contributed equally to this manuscript and should be regarded as co-corresponding authors; all authors issued final approval for the version to be submitted.
Supported by the Clinical Research Foundation of the Third Affiliated Hospital of Sun Yat-Sen University, No. YHJH201904; and National Science and Technology Major Project, No. 2018ZX10302204.
Informed consent statement: Informed written consent was obtained from the patient’s father for publication of this report and any accompanying images.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
CARE Checklist (2016) statement: The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Zhi-Liang Gao, BMed, MD, PhD, Chief Physician, Professor, Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China. gaozhl@mail.sysu.edu.cn
Received: February 17, 2021
Peer-review started: February 17, 2021
First decision: March 11, 2021
Revised: March 31, 2021
Accepted: April 20, 2021
Article in press: April 20, 2021
Published online: July 16, 2021
Processing time: 139 Days and 23.5 Hours
Core Tip

Core Tip: Mycobacterium mucogenicum (M. mucogenicum) belongs to the group of Nontuberculous mycobacteria, and is associated with a wide spectrum of clinical diseases, including osteomyelitis, respiratory tract, bloodstream, and disseminated infections in both immunocompetent and immunosuppressed individuals. However, time-consuming techniques and a low detection rate for identifying this pathogen usually lead to delayed or missed diagnosis. We present a case of disseminated infection with M. mucogenicum diagnosed in a formalin-fixed, paraffin-embedded (FFPE) specimen using next-generation sequencing (NGS). NGS is a promising tool for the rapid and accurate diagnosis of rare pathogens, even when using a FFPE specimen.