Retrospective Cohort Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Apr 26, 2023; 11(12): 2684-2693
Published online Apr 26, 2023. doi: 10.12998/wjcc.v11.i12.2684
Analysis of oxidative stress and antioxidative potential in premature ovarian insufficiency
Kaoru Kakinuma, Toshiyuki Kakinuma
Kaoru Kakinuma, Toshiyuki Kakinuma, Department of Obstetrics and Gynecology, International Health and Welfare Hospital, Nasushiobara 327-2763, Japan
Author contributions: Kakinuma K and Kakinuma T contributed to the methodology, software design, validation, and formal analysis, writing-original draft preparation, writing-review and editing, visualization, supervision, and project administration.
Institutional review board statement: The present study was approved by the Institutional Review Board of the International Health and Welfare Hospital (approval No. 21-Im-075, approved on 3/22/2022).
Informed consent statement: All study participants or their legal guardian provided informed written consent about personal and medical data collection prior to study enrolment.
Conflict-of-interest statement: All the Authors have no conflict of interest related to the manuscript.
Data sharing statement: The original anonymous dataset is available on request from the corresponding author at tokakinuma@gmail.com.
STROBE statement: The authors have read the STROBE Statement—checklist of items, and the manuscript was prepared and revised according to the STROBE Statement—checklist of items.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Toshiyuki Kakinuma, MD, PhD, Professor, Department of Obstetrics and Gynecology, International Health and Welfare Hospital, 537-3, Iguchi, Nasushiobara 327-2763, Japan. tokakinuma@gmail.com
Received: January 6, 2023
Peer-review started: January 6, 2023
First decision: January 30, 2023
Revised: February 20, 2023
Accepted: March 23, 2023
Article in press: March 23, 2023
Published online: April 26, 2023
Processing time: 109 Days and 13.7 Hours
ARTICLE HIGHLIGHTS
Research background

Premature ovarian insufficiency (POI) is characterized by the premature decline of ovarian function, inducing secondary amenorrhea and leading to severe infertility. Excessive production of reactive oxygen species (ROS) induces DNA damage, lipid peroxidation, and protein denaturation, while oxidative stress causes or exacerbates various diseases. The function of mitochondria in the ovaries and the cytotoxicity associated with ROS have been implicated in follicle pool depletion and follicle quality decline. Diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) can be easily measured, and tests have been developed for the comprehensive evaluation of blood oxidative stress by combining the d-ROMs and BAP tests.

Research motivation

Most cases of POI are idiopathic, and no definitive cause has yet been identified. Investigation of the cause of POI, early diagnosis, and early intervention are warranted.

Research objectives

This study sought to comprehensively assess the oxidative stress status with d-ROMs and BAP tests in POI and to investigate whether these can be biomarkers for POI.

Research methods

To comprehensively assess oxidative stress status, we measured plasma d-ROM and BAP in POI and control groups.

Research results

The d-ROMs level and the oxidase stress index were significantly higher in the POI than in the control group. However, the BAP level did not significantly differ between the two groups.

Research conclusions

Oxidative stress (d-ROMs, OSI) in the POI group was significantly higher than in the control group, suggesting that the oxidative stress state may be a factor in POI and a potential biomarker. Therefore, it may be useful for early intervention for treatment, including infertility treatment.

Research perspectives

Oxidative stress was significantly higher in patients with POI than in healthy controls, suggesting the use of this measurement as a biomarker of POI. In the future, we plan to investigate whether these markers are useful for the early diagnosis of POI and how the state of oxidative stress affects the pathology of POI.