Case Report
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Dec 26, 2020; 8(24): 6465-6472
Published online Dec 26, 2020. doi: 10.12998/wjcc.v8.i24.6465
Novel mutation in the ASXL3 gene in a Chinese boy with microcephaly and speech impairment: A case report
Jin-Rong Li, Zhuo Huang, You Lu, Qiao-Yun Ji, Ming-Yan Jiang, Fan Yang
Jin-Rong Li, Zhuo Huang, You Lu, Qiao-Yun Ji, Ming-Yan Jiang, Fan Yang, Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
Jin-Rong Li, Ming-Yan Jiang, Fan Yang, Department of Ministry of Education, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Chengdu 610041, Sichuan Province, China
Author contributions: Yang F and Jiang MY conceived the study and its design; Huang Z performed literature research; Li JR and Lu Y were involved in the clinical studies; Ji QY performed data and statistical analyses; Li JR was involved in manuscript preparation and manuscript review; Jiang MY and Huang Z edited the manuscript; all authors have read and approved this article.
Supported by the Health and Family Planning Commission of Sichuan Province, No. 150106.
Informed consent statement: Informed written consent was obtained from the patient’ parent for publication of this report and any accompanying images.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
CARE Checklist (2016) statement: The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Ming-Yan Jiang, MD, Physiotherapist, Department of Pediatrics, West China Second University Hospital, Sichuan University, No. 20 Section 3, South Renmin Road, Chengdu 610041, Sichuan Province, China. jiangmy0904@qq.com
Received: August 28, 2020
Peer-review started: August 28, 2020
First decision: September 24, 2020
Revised: October 8, 2020
Accepted: October 26, 2020
Article in press: October 26, 2020
Published online: December 26, 2020
Processing time: 113 Days and 13.5 Hours
Abstract
BACKGROUND

Bainbridge-Ropers syndrome (BRPS) is a severe disorder characterized by failure to thrive, facial dysmorphism, and severe developmental delay. BRPS is caused by a heterozygous loss-of-function mutation in the ASXL3 gene. Due to limited knowledge of the disease and lack of specific features, clinical diagnosis of this syndrome is challenging. With the use of trio-based whole exome sequencing, we identified a novel ASXL3 mutation in a Chinese boy with BRPS and performed a literature review.

CASE SUMMARY

A 3-year-old Chinese boy was referred to our hospital due to progressive postnatal microcephaly and intellectual disability with severe speech impairment for 2 years. His other remarkable clinical features were shown as follows: Facial dysmorphism, feeding difficulties, poor growth, motor delay, and abnormal behavior. For the proband, regular laboratory tests, blood tandem mass spectrometry, urine gas chromatographic mass spectrometry, karyotype, hearing screening, and brain magnetic resonance imaging were performed, with negative results. Therefore, for the proband and his unaffected parents, trio-based whole exome sequencing and subsequent validation by Sanger sequencing were performed. A novel nonsense variant in exon 11 of the ASXL3 gene (c.1795G>T; p.E599*) was detected, present in the patient but absent from his parents. Taking into account the concordant phenotypic features of our patient with reported BRPS patients and the detected truncated variant located in the known mutational cluster region, we confirmed a diagnosis of BRPS for this proband. The rehabilitation treatment seemed to have a mild effect.

CONCLUSION

In this case, a novel nonsense mutation (c.1795G>T, p.E599*) in ASXL3 gene was identified in a Chinese boy with BRPS. This finding not only contributed to better genetic counseling and prenatal diagnosis for this family but also expanded the pathogenic mutation spectrum of ASXL3 gene and provided key information for clinical diagnosis of BRPS.

Keywords: Bainbridge-Ropers syndrome; ASXL3 mutation; Whole-exome sequencing; Case report

Core Tip: This report introduces a Chinese boy referred to our hospital mainly due to progressive postnatal microcephaly and intellectual disability with severe speech impairment. A novel pathogenic mutation (c.1795G>T; p.E599*) was detected in the patient by trio-based whole exome sequencing. The proband’s clinical features largely conformed to reported Bainbridge-Ropers syndrome patients in the literature. These clinical and genetic findings improved our understanding of Bainbridge-Ropers syndrome and also aided in the definitive diagnosis and genetic counseling for this family.