Retrospective Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Jan 14, 2022; 10(2): 469-476
Published online Jan 14, 2022. doi: 10.12998/wjcc.v10.i2.469
Relationship between Ki-67 and CD44 expression and microvascular formation in gastric stromal tumor tissues
Bing Ma, Xiao-Tian Huang, Gui-Jun Zou, Wen-Yu Hou, Xiao-Hui Du
Bing Ma, Xiao-Tian Huang, Gui-Jun Zou, Wen-Yu Hou, Xiao-Hui Du, Department of General Surgery, The PLA General Hospital, Beijing 100853, China
Author contributions: Ma B and Du XH performed the research; Huang XT contributed new reagents and analytic tools; Zou GJ and Hou WY analyzed the data and wrote the manuscript; all authors have read and approve the final manuscript.
Institutional review board statement: The study was reviewed and approved by the PLA General Hospital Institutional Review Board (Approval No. 20210321).
Informed consent statement: All study participants provided informed written consent prior to study enrollment.
Conflict-of-interest statement: None conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Xiao-Hui Du, MD, Chief Physician, Department of General Surgery, The PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing 100853, China. duxiaohui301@sina.com
Received: August 26, 2021
Peer-review started: August 26, 2021
First decision: September 29, 2021
Revised: October 8, 2021
Accepted: November 29, 2021
Article in press: November 29, 2021
Published online: January 14, 2022
Processing time: 138 Days and 16.9 Hours
Abstract
BACKGROUND

A gastric stromal tumor (GST) is a mesenchymal tumor that occurs in the gastrointestinal tract; its biological characteristics are highly complex. Clinically, the severity of a GST is often evaluated by factors such as risk classification, tumor size, and mitotic figures. However, these indicators are not very accurate. Even patients classified as low risk are also at risk of metastasis and recurrence. Therefore, more accurate and objective clinical biological behavior evaluations are urgently needed.

AIM

To determine the relationship between Ki-67 and CD44 expression in GSTs and microvessel formation and prognosis.

METHODS

Eighty-six GST tissue specimens from our hospital were selected for this study. The immunohistochemical staining technique was used to detect Ki-67, CD44, and microvessel density (MVD) in the collected samples to analyze the different risk grades and mitotic figures. In addition, this approach was used to determine the differences in the expression of Ki-67 and CD44 in GST tissues with varying lesion diameters.

RESULTS

In GSTs with positive expression of the Ki-67 protein, the proportions of patients with medium-to-high risk and more than five mitotic counts were 24.07% and 38.89%, respectively. In GSTs with positive expression of the CD44 protein, the proportions of patients with medium-to-high risk and more than five mitotic counts were 23.73% and 38.98%, respectively. In GSTs with negative expression of the Ki-67 protein, these values were relatively high (3.70% and 11.11%, respectively). The MVD in GSTs with positive and negative expression of the CD44 protein was 15.92 ± 2.94 and 13.86 ± 2.98/Hp, respectively; the difference between the two groups was significant (P < 0.05).

CONCLUSION

Ki-67 and CD44 expression in GSTs is correlated with the grade of tumor risk and mitotic figures. CD44 expression is correlated with microvessel formation in tumor tissues.

Keywords: Gastric stromal tumor; Ki-67; CD44; Expression; Microvascular formation; Formation of microvessels

Core Tip: Microvascular plays a key role in the occurrence and development of gastric stromal tumor. Through this study, we reveal its role in tumor metastasis and invasion, and provide a basis for predicting the clinical prognosis of patients.