Improved method for inducing chronic atrophic gastritis in mice

Table 1 Identification of cultures isolated from gastric mucosa of mice in the combined group based on biochemical indices

Group	Oxidase	Peroxidase	Urease
Positive control	+	+	+
Negative control	-	-	-
Isolated culture	+	+	+

Table 2 Immunological functionsof mice in the combined group

Group	CD4+			CD8+
	30th d	90th d	120th d	30th d	90th d	120th d
PBS control	52.56 ± 1.82	51.27 ± 2.05	52.84 ± 1.49	28.13 ± 1.92	29.41 ± 2.39	30.07 ± 1.24
Combined	40.17 ± 1.36	44.38 ± 2.11	43.97 ± 2.08	22.08 ± 1.42	25.53 ± 1.87	25.19 ± 1.53

Table 3 Modelling results of chronic atrophic gastritis

Group	The number of mice developing CAG		The number of mice with Hp colonisation		Success rate
	90th d after infection	120th d after infection	90th d after infection	120th d after infection
PBS control	0	0	0	0	0
Hp	0	0	90% (9/10)	100% (10/10)	0
MNNG	0	0	0	0	0
Ammonia water	0	0	0	0	0
Combined	90% (9/10)	90 (9/10)	100% (10/10)	100% (10/10)	90%

CAG: chronic atrophic gastritis; MNNG: N-methyl-N'-nitroguanidine.