Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. May 15, 2019; 11(5): 377-392
Published online May 15, 2019. doi: 10.4251/wjgo.v11.i5.377
Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways
Hong Yang, Jian-Xin Liu, Hai-Xia Shang, Shan Lin, Jin-Yan Zhao, Jiu-Mao Lin
Hong Yang, Jian-Xin Liu, Hai-Xia Shang, Shan Lin, Jin-Yan Zhao, Jiu-Mao Lin, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China
Hong Yang, Jian-Xin Liu, Hai-Xia Shang, Shan Lin, Jin-Yan Zhao, Jiu-Mao Lin, Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China
Author contributions: Lin JM and Yang H conceived and designed the experiments; Yang H, Liu JX and Shang HX conducted MTT, LDH, Hoechst 33258 and western blot assays and analysis; Lin S and Zhao JY conducted fluorescence-activated cell sorting and analysis; Yang H and Lin JM wrote the manuscript. All authors are responsible for the obtainment of written permission to use any copyrighted text and/or illustrations.
Institutional review board statement: This study was reviewed and approved by Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine.
Conflict-of-interest statement: The authors declare no conflicts of interest in the present study.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Jiu-Mao Lin, PhD, Senior Research Fellow, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, 1 Qiuyang Road, Minhou Shangjie, Fuzhou 350122, Fujian Province, China. linjiumao@fjtcm.edu.cn
Supported by Project Funding of the Scientific Research Foundation of Traditional Chinese Medicine of Fujian Provincial Health and Family Planning Commission, No. 2017FJZYZY203; and the Training of Young and Middle-Aged Backbone Personnel of Fujian Provincial Health and Family Planning Commission, No. 2016-ZQN-67/.
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Received: November 20, 2018
Peer-review started: November 20, 2018
First decision: December 7, 2018
Revised: December 17, 2018
Accepted: January 3, 2019
Article in press: January 4, 2019
Published online: May 15, 2019
Processing time: 176 Days and 20.7 Hours
Core Tip

Core tip: To study the effect and mechanism of Qingjie Fuzheng granules (QFGs) on colorectal cancer (CRC) cells, we measured cell viability and cytotoxicity by performing MTT and LDH assays. We also evaluated the role of QFGs in cell proliferation by conducting colony formation, cell cycle and western blot assays. We evaluated the role of QFGs in cell apoptosis by assessing both Hoechst 33258 and Annexin-V/PI staining and performing western blot assays. Furthermore, the activation of PI3K/AKT and ERK signaling pathways was examined using the western blot assay to investigate the possible mechanism. All results demonstrated that QFGs inhibited CRC cell growth by suppressing the PI3K/AKT and ERK signaling pathways.