Basic Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Sep 15, 2023; 15(9): 1556-1566
Published online Sep 15, 2023. doi: 10.4251/wjgo.v15.i9.1556
MicroRNA-363-3p inhibits colorectal cancer progression by targeting interferon-induced transmembrane protein 1
Yun Wang, Shao-Kai Bai, Tao Zhang, Cheng-Gong Liao
Yun Wang, Shao-Kai Bai, Tao Zhang, Cheng-Gong Liao, Department of Oncology, Tangdu Hospital, Air Force Medical University, Xi’an 710038, Shaanxi Province, China
Author contributions: Wang Y and Bai SK designed and performed the assay; Zhang T analyzed the data; Liao CG designed the study and prepared the manuscript.
Supported by the Social Talent Fund Supporting Scheme of Tangdu Hospital, No. 2021SHRC001.
Institutional review board statement: The study was approved by the Hospital Ethics Committee (202203-116).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Cheng-Gong Liao, PhD, Chief Physician, Department of Oncology, Tangdu Hospital, Air Force Medical University, No. 569 Xinsi Road, Xi’an 710038, Shaanxi Province, China.
Received: May 5, 2023
Peer-review started: May 5, 2023
First decision: July 9, 2023
Revised: July 21, 2023
Accepted: August 18, 2023
Article in press: August 18, 2023
Published online: September 15, 2023
Research background

Colorectal cancer is the second most common cause of cancer death, however, the molecular mechanisms of tumorigenesis and development of colorectal cancer are far from being elucidated.

Research motivation

MicroRNAs play important roles in gene regulation and modulate numerous physical and pathological processes. The motivation of this study is to reveal the role of microRNA-363-3p (miR-363-3p) in the development of colorectal cancer and the underlying mechanisms.

Research objectives

Compare the expression of miR-363-3p between colorectal cancer tissues and adjacent normal tissues; clarify the role of miR-363-3p in clonogenic survival, migration, and proliferation of colorectal cancer cells; identify the direct target of miR-363-3p in colorectal cancer cells.

Research methods

Real-time polymerase chain reaction was performed to detect miRNA expression. PITA 6 was utilized to predict the targets of miR-363-3p. Dual-luciferase reporter system was used to validate the target of miR-363-3p. Plate colony formation and wound-healing assays were performed to evaluate cancer cells’ clonogenic survival and migration ability, respectively. Cell proliferation was examined by cell counting kit-8 assay. Immunohistochemical staining was used to determine the expression level of interferon-induced transmembrane protein 1 (IFITM1).

Research results

MiR-363-3p was decreased in colorectal cancer tissues. IFITM1 was characterized as a direct target of miR-363-3p.

Research conclusions

MiR-363-3p inhibits clonogenic survival, proliferation, and migration of colorectal cancer cells via targeting IFITM1.

Research perspectives

MiR-363-3p/IFITM1 axis may represent a therapeutic target in colorectal cancer.