Cai XX, Huang ZF, Tu FY, Yu J. Impact and mechanism study of dioscin on biological characteristics of colorectal cancer cells. World J Gastrointest Oncol 2024; 16(11): 4456-4467 [PMID: 39554744 DOI: 10.4251/wjgo.v16.i11.4456]
Corresponding Author of This Article
Jie Yu, BMed, Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Wenzhou Medical University, No. 108 Wansong Road, Wenzhou 325200, Zhejiang Province, China. 15968760998@163.com
Research Domain of This Article
Oncology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastrointest Oncol. Nov 15, 2024; 16(11): 4456-4467 Published online Nov 15, 2024. doi: 10.4251/wjgo.v16.i11.4456
Impact and mechanism study of dioscin on biological characteristics of colorectal cancer cells
Xie-Xiao Cai, Zhen-Feng Huang, Fu-Yang Tu, Jie Yu
Xie-Xiao Cai, Zhen-Feng Huang, Fu-Yang Tu, Jie Yu, Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou 325200, Zhejiang Province, China
Author contributions: Cai XX and Yu J initiated research and conducted data collection; Huang ZF and Tu FY recorded, organized data, and conducted statistical analysis; Cai XX and Yu J wrote the original manuscript and revised the paper; all authors read and approved the final manuscript.
Institutional review board statement: This study did not involve any human or animal experiment.
Conflict-of-interest statement: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Data sharing statement: All data generated or analyzed during this study are included in this published article.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Jie Yu, BMed, Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Wenzhou Medical University, No. 108 Wansong Road, Wenzhou 325200, Zhejiang Province, China. 15968760998@163.com
Received: August 1, 2024 Revised: September 11, 2024 Accepted: September 29, 2024 Published online: November 15, 2024 Processing time: 85 Days and 2.3 Hours
Abstract
BACKGROUND
Colorectal cancer (CRC) is a considerable global health issue. Dioscin, a compound found in several medicinal plants, has shown potential anticancer effects.
AIM
To find the relationship between CRC cells (HCT116) and diosgenin and clarified their mechanisms of action.
METHODS
CRC cell line HCT116 was cultured by dividing cells into control and dioscin groups (dioscin + Jagged 1 group; Jagged 1 group, 5 μg/mL; and dioscin group, 2.5 μg/mL). The dioscin groups were given different concentrations of dioscin. Cell Counting Kit-8 was chosen for testing cell viability in different groups. Flow cytometry was established to undiscover the apoptosis rate of human liver cancer cell line 11. Real-time PCR as well as Western blot analyses were applied to reveal the expression levels of caspase-3, Notch, and other proteins. Transwell and scratch experiments were conducted to assess cell migration and invasion abilities.
RESULTS
This study indicated that dioscin restricted the growth of HCT116 cells, boosted cell apoptosis, and rose the Bax/Bcl-2 ratio as well as the expression of Caspase-3. Dioscin also inhibited physiological activities, for instance cell migration, and significantly reduced the expression levels of proteins for instance Notch1 (P < 0.05). Dioscin partially reversed the effects of Jagged 1.
CONCLUSION
Dioscin exerts a certain inhibitory effect on HCT116, and its mechanism of action may be linked, with the inhibition of the Notch1 signaling pathway.
Core Tip: This study investigates the anticancer effects of dioscin on colorectal cancer (CRC) cells (HCT116). Our findings reveal that dioscin inhibits cell viability, promotes apoptosis, and suppresses migration and invasion by modulating the Notch1 signaling pathway. These results highlight the potential of dioscin as a therapeutic agent against CRC.
