Weng JS, Huang JP, Yu W, Xiao J, Lin F, Lin KN, Zang WD, Ye Y, Lin JP. Mitophagy-related gene signature predicts prognosis, immune infiltration and chemotherapy sensitivity in colorectal cancer. World J Gastrointest Oncol 2023; 15(3): 546-561 [PMID: 37009318 DOI: 10.4251/wjgo.v15.i3.546]
Corresponding Author of This Article
Jing-Ping Lin, MD, Doctor, Critical Care Medicine, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, No. 420 Fuma Road, Fuzhou 350014, Fujian Province, China. ljpccm@163.com
Research Domain of This Article
Oncology
Article-Type of This Article
Retrospective Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastrointest Oncol. Mar 15, 2023; 15(3): 546-561 Published online Mar 15, 2023. doi: 10.4251/wjgo.v15.i3.546
Mitophagy-related gene signature predicts prognosis, immune infiltration and chemotherapy sensitivity in colorectal cancer
Jin-Sen Weng, Jie-Ping Huang, Wei Yu, Jun Xiao, Fang Lin, Kang-Ni Lin, Wei-Dong Zang, Yong Ye, Jing-Ping Lin
Jin-Sen Weng, Fang Lin, Kang-Ni Lin, Yong Ye, Jing-Ping Lin, Critical Care Medicine, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, Fujian Province, China
Jie-Ping Huang, Department of Emergency, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China
Wei Yu, Clinical Pharmacy, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, Fujian Province, China
Jun Xiao, Wei-Dong Zang, Gastrointestinal Surgery Department, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, Fujian Province, China
Author contributions: Weng JS, Huang JP, and Yu W contributed equally to this work; Lin JP and Ye Y had the idea for this study; Weng JS, Huang JP, and Yu W supervised the acquisition of the data; Xiao J and Zang WD undertook the statistical analysis; Lin F, Lin KN, and Zang WD provided statistical advice; all authors contributed to interpretation of the results; Weng JS, Huang JP, and Yu W wrote the article and other authors contributed to the content; all authors approved the final version of the manuscript, including the authorship list.
Institutional review board statement: The study was reviewed and approved by the Fujian Cancer Ethics Committee (Approval No. K2023-030-01).
Informed consent statement: All the data were downloaded from GEO and TCGA database, thus the informed consent statement is not applicable in this study.
Conflict-of-interest statement: The authors declare that they have no competing interests.
Data sharing statement: Source data and reagents are available from the corresponding author upon reasonable request.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Jing-Ping Lin, MD, Doctor, Critical Care Medicine, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, No. 420 Fuma Road, Fuzhou 350014, Fujian Province, China. ljpccm@163.com
Received: November 20, 2022 Peer-review started: November 20, 2022 First decision: December 10, 2022 Revised: December 23, 2022 Accepted: February 23, 2023 Article in press: February 23, 2023 Published online: March 15, 2023 Processing time: 114 Days and 13.6 Hours
Abstract
BACKGROUND
Mitophagy plays essential role in the development and progression of colorectal cancer (CRC). However, the effect of mitophagy-related genes in CRC remains largely unknown.
AIM
To develop a mitophagy-related gene signature to predict the survival, immune infiltration and chemotherapy response of CRC patients.
METHODS
Non-negative matrix factorization was used to cluster CRC patients from Gene Expression Omnibus database (GSE39582, GSE17536, and GSE37892) based on mitophagy-related gene expression. The CIBERSORT method was applied for the evaluation of the relative infiltration levels of immune cell types. The performance signature in predicting chemotherapeutic sensitivity was generated using data from the Genomics of Drug Sensitivity in Cancer database.
RESULTS
Three clusters with different clinicopathological features and prognosis were identified. Higher enrichment of activated B cells and CD4+ T cells were observed in cluster III patients with the most favorable prognosis. Next, a risk model based on mitophagy-related genes was developed. Patients in training and validation sets were categorized into low-risk and high-risk subgroups. Low risk patients showed significantly better prognosis, higher enrichment of immune activating cells and greater response to chemotherapy (oxaliplatin, irinotecan, and 5-fluorouracil) compared to high-risk patients. Further experiments identified CXCL3 as novel regulator of cell proliferation and mitophagy.
CONCLUSION
We revealed the biological roles of mitophagy-related genes in the immune infiltration, and its ability to predict patients’ prognosis and response to chemotherapy in CRC. These interesting findings would provide new insight into the therapeutic management of CRC patients.
Core Tip: We for the first time revealed the biological roles of mitophagy-related genes in the immune infiltration, and its prediction of patients’ prognosis and chemotherapy response in colorectal cancer. These novel findings would provide new insight into the therapeutic management of colorectal cancer patients.
