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©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. May 26, 2023; 15(5): 466-475
Published online May 26, 2023. doi: 10.4252/wjsc.v15.i5.466
Published online May 26, 2023. doi: 10.4252/wjsc.v15.i5.466
Single-cell RNA sequencing in cornea research: Insights into limbal stem cells and their niche regulation
Di Sun, Wei-Yun Shi, Sheng-Qian Dou, State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Eye Institute of Shandong First Medical University, Qingdao 266000, Shandong Province, China
Di Sun, Sheng-Qian Dou, Qingdao Eye Hospital of Shandong First Medical University, Qingdao 266000, Shandong Province, China
Wei-Yun Shi, Eye Hospital of Shandong First Medical University, Jinan 250000, Shandong Province, China
Wei-Yun Shi, School of Ophthalmology, Shandong First Medical University, Qingdao 266000, Shandong Province, China
Author contributions: Dou SQ and Shi WY designed the report; Sun D collected the data and wrote the paper; Dou SQ and Shi WY reviewed and edited the manuscript; and all authors discussed the study’s results and provided important intellectual comments on the manuscript.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Sheng-Qian Dou, PhD, Associate Professor, State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Eye Institute of Shandong First Medical University, No. 5 Yan’erdao Road, Qingdao 266000, Shandong Province, China. doushq@126.com
Received: December 30, 2022
Peer-review started: December 30, 2022
First decision: March 14, 2023
Revised: March 28, 2023
Accepted: April 17, 2023
Article in press: April 17, 2023
Published online: May 26, 2023
Processing time: 147 Days and 5.8 Hours
Peer-review started: December 30, 2022
First decision: March 14, 2023
Revised: March 28, 2023
Accepted: April 17, 2023
Article in press: April 17, 2023
Published online: May 26, 2023
Processing time: 147 Days and 5.8 Hours
Core Tip
Core Tip: Limbal stem cells (LSCs), a cell population that resides at the limbus in a highly regulated niche. With the advent of single-cell RNA sequencing, our understanding of LSC characteristics and their microenvironment has grown considerably. This review focuses on the current research on single cell sequencing in LSCs. We highlight the heterogeneity, novel specific markers and niche regulation of LSCs.