Basic Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Nov 26, 2022; 14(11): 798-814
Published online Nov 26, 2022. doi: 10.4252/wjsc.v14.i11.798
Tumor necrosis factor-α inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation
Jiang-Tao Wan, Xian-Shuai Qiu, Zhuo-Hang Fu, Yong-Can Huang, Shao-Xiong Min
Jiang-Tao Wan, Zhuo-Hang Fu, Yong-Can Huang, Shao-Xiong Min, Department of Spine Surgery, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong Province, China
Jiang-Tao Wan, Zhuo-Hang Fu, Yong-Can Huang, Institute of Orthopedics, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen 518036, Guangdong Province, China
Xian-Shuai Qiu, Shao-Xiong Min, Department of Spine Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China
Author contributions: Min SX and Wan JT conceived and designed the experiments; Wan JT, Qiu XS, and Fu ZH performed the experiments; Wan JT and Qiu XS analyzed the data; Wan JT and Huang YC composed the manuscript.
Supported by the Natural Science Foundation of Guangdong Province, China, No. 2017A030313564; and Shenzhen High-level Hospital Construction Fund to Peking University Shenzhen Hospital.
Institutional review board statement: This study was approved by the Ethics Committee of Peking University Shenzhen Hospital (No. 2022-076).
Conflict-of-interest statement: The author has no conflict of interest to declare.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Shao-Xiong Min, PhD, Chief Physician, Department of Spine Surgery, Peking University Shenzhen Hospital, No. 1120 Lianhua Road, Futian District, Shenzhen 518036, Guangdong Province, China. msxbear24@163.com
Received: July 20, 2022
Peer-review started: July 20, 2022
First decision: August 8, 2022
Revised: October 5, 2022
Accepted: November 22, 2022
Article in press: November 22, 2022
Published online: November 26, 2022
Processing time: 128 Days and 2.3 Hours
Core Tip

Core Tip: Adipose stem cells are important seed cells that are used in cartilage tissue engineering. However, at present, cartilage matrix secretion by adipose-derived stem cells (ADSCs) inevitably decreases during the late stage of induced chondrogenic differentiation in vitro, which seriously limits the further application of ADSCs. Our team found that the level of inflammation in the culture system, mainly the levels of tumor necrosis factor-alpha (TNF-α) and matrix metalloproteinase 3, continuously increased during the chondrogenic differentiation of ADSCs. To address this issue, our team added etanercept or infliximab, which are targeted inhibitors of TNF-α, to the chondrogenic differentiation induction medium and successfully restored matrix formation by human ADSCs in the late stage of chondrogenic differentiation. Further studies found that these effects were achieved by reducing NF-κB pathway activation.