Published online Sep 26, 2023. doi: 10.4252/wjsc.v15.i9.947
Peer-review started: August 14, 2023
First decision: August 22, 2023
Revised: August 31, 2023
Accepted: September 14, 2023
Article in press: September 14, 2023
Published online: September 26, 2023
Hemostasis and wound healing are one of the common problems in clinics which need to be paid attention to. Gelatin sponge is often used as a hemostatic material in clinics. Exosomes have been proved to play an important role in wound repair. Therefore, it is worth studying whether the combination of exosomes derived from human umbilical cord mesenchymal stem cells (hUC-MSCs) and a gelatin sponge could promote hemostasis and wound healing more efficiently.
Poor wound healing would contribute a negative impact on patients’ quality of life and aggravate pain, stress and depression, so it is of great significance to find ways to effectively promote wound healing.
The present study aimed to investigate the hemostatic and wound healing efficacy of a gelatin sponge loaded with hUC-MSCs-derived exosomes.
After the exosomes were extracted and characterized by ultracentrifugation, we loaded the exosomes on a gelatin sponge. Then, in vitro and in vivo experiments, including cell viability assay, hemolysis assay, skin irritation test, and histocompatibility assay were performed to verify the safety of the exosome-loaded gelatin sponge. Subsequently, whole blood coagulation index test, hemostatic assay using a rat liver defect hemostasis model, and full-thickness skin defect healing promoting test were performed to verify the effects of the exosome-loaded gelatin sponge in hemostasis and wound healing.
We successfully extracted exosomes from hUC-MSCs. The safety experiments showed that the gelatin sponge loaded with exosomes would not cause abnormal proliferation of L929 cells, hemolysis, or irritation to skin and tissues. In addition, the exosome-loaded gelatin sponge had a better hemostatic effect than the traditional gelatin sponge, which can promote the formation of collagen fibers and blood vessels around the wound and increase the proportion of Ki67-positive cells, thus promoting the wound healing.
In a word, gelatin sponge loaded with hUC-MSCs-derived exosomes is safe. It is better than traditional gelatin sponge in stopping bleeding and promoting wound healing.
The gelatin sponge loaded with exosomes derived from hUC-MSCs may be a potential material to stop bleeding and promote wound healing.