Published online May 26, 2023. doi: 10.4252/wjsc.v15.i5.490
Peer-review started: November 9, 2022
First decision: December 25, 2022
Revised: January 21, 2023
Accepted: April 12, 2023
Article in press: April 12, 2023
Published online: May 26, 2023
Stromal cell-derived factor-1α (SDF-1α) has a chemotactic effect on mesenchymal stem cells (MSCs), and SDF-1α and MSCs are used together to treat cartilage degeneration and cartilage defects. The specific effects of SDF-1α on cartilage differentiation in MSCs need to be clarified.
Understanding the effects of SDF-1α on MSCs will provide a new theoretical basis for the use of MSCs in the repair of cartilage degeneration.
To explore the role and mechanism of SDF-1α on cartilage differentiation in MSCs and primary chondrocytes.
MSCs were treated with SDF-1α and subsequently stained for alkaline phosphatase and with Alcian blue to demonstrate chondrogenic differentiation. Western blot analysis was used to examine the expression of cartilage differentiation-related and Wnt/β-catenin pathway proteins in MSCs and primary chondrocytes.
After extraction and incubation with the appropriate differentiation media, MSCs differentiated into the three skeletal lineages. SDF-1α exerted no effect on early cartilage formation but enhanced hypertrophic differentiation in MSCs. SDF-1α had no effect on the expression of SRY-box transcription factor 9, aggrecan, and collagen II but increased the expression of runx family transcription factor 2, collagen X, and matrix metalloproteinase 13 in MSCs and primary chondrocytes. SDF-1α increased the expression of p- glycogen synthase kinase 3β and β-catenin.
SDF-1α enhanced hypertrophic differentiation in MSCs and primary chondrocytes. This effect was achieved by activating the Wnt/β-catenin pathway.
These findings provide a new theoretical basis for the treatment of cartilage degeneration with MSCs.