Garrigós MM, de Oliveira FA, Nucci MP, Nucci LP, Alves ADH, Dias OFM, Gamarra LF. How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models. World J Stem Cells 2022; 14(8): 658-679 [PMID: 36157912 DOI: 10.4252/wjsc.v14.i8.658]
Corresponding Author of This Article
Lionel Fernel Gamarra, PhD, Academic Research, Full Professor, Research Scientist, Hospital Israelita Albert Einstein, Av Albert Einstein 640, São Paulo 05652-900, São Paulo, Brazil. lionelgamarra7@gmail.com
Research Domain of This Article
Hematology
Article-Type of This Article
Systematic Reviews
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Stem Cells. Aug 26, 2022; 14(8): 658-679 Published online Aug 26, 2022. doi: 10.4252/wjsc.v14.i8.658
How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models
Murilo Montenegro Garrigós, Fernando Anselmo de Oliveira, Mariana Penteado Nucci, Leopoldo Penteado Nucci, Arielly da Hora Alves, Olivia Furiama Metropolo Dias, Lionel Fernel Gamarra
Murilo Montenegro Garrigós, Fernando Anselmo de Oliveira, Mariana Penteado Nucci, Arielly da Hora Alves, Olivia Furiama Metropolo Dias, Lionel Fernel Gamarra, Hospital Israelita Albert Einstein, São Paulo 05652-900, São Paulo, Brazil
Murilo Montenegro Garrigós, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, São Paulo, Brazil
Mariana Penteado Nucci, LIM44-Hospital das Clínicas, Faculdade Medicina da Universidade de São Paulo, São Paulo 05403-000, Brazil
Leopoldo Penteado Nucci, Centro Universitário do Planalto Central, Área Especial para Industria nº 02 Setor Leste - Gama-DF, Brasília 72445-020, Distrito Federal, Brazil
Author contributions: Garrigós MM, de Oliveira FA and Gamarra LF, conceptualized and designed the review; de Oliveira FA, and Garrigós MM contributed to search of literature, data extraction and critical revision; Nucci MP, Nucci LP, Dias OFM, Alves AH and Gamarra LF carried out the analysis, drafted the manuscript and critical revision; all authors reviewed and approved the final manuscript as submitted.
Supported byCNPq, No. 308901/2020, No. 400856/2016-6; FAPESP, No. 2019/21070-3, No. 2017/17868-4, No. 2016/21470-3; SisNANO 2.0/MCTIC, No. 442539/2019-3; the National Institute of Science and Technology Complex Fluids (INCT-FCx); and “Amigos da Oncologia e Hematologia Einstein” AMIGOH.
Conflict-of-interest statement: The authors have declared that no competing interests exist.
PRISMA 2009 Checklist statement: The authors have read the PRISMA guideline and the manuscript was prepared and revised according to the PRISMA 2009 Checklist.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Lionel Fernel Gamarra, PhD, Academic Research, Full Professor, Research Scientist, Hospital Israelita Albert Einstein, Av Albert Einstein 640, São Paulo 05652-900, São Paulo, Brazil. lionelgamarra7@gmail.com
Received: March 15, 2022 Peer-review started: March 15, 2022 First decision: April 19, 2022 Revised: April 27, 2022 Accepted: July 26, 2022 Article in press: July 26, 2022 Published online: August 26, 2022 Processing time: 163 Days and 16.9 Hours
ARTICLE HIGHLIGHTS
Research background
Although bone marrow transplantation (BMT) may be applied to the treatment of hematological and nonhematological diseases, this treatment still presents a series of difficulties and obstacles that corroborate to the treatment failure.
Research motivation
The motivation to study the use of mesenchymal stem cells (MSCs) in hematopoietic stem cell (HSC) transplantation is that the use of both cells at once may increase the success rate of BMT.
Research objectives
The purpose of this systematic review was to investigate the characteristics of HSCs and MSCs, as well as their various interactions in murine models of cotransplantation.
Research methods
A systematic review was conducted in the PubMed and Scopus databases, looking for original articles from the last decade that used HSC and MSC cotransplantation, as well as in vivo BMT in animal models, excluding studies involving graft-versus-host disease or other diseases.
Research results
Only 18 of 2565 articles found in the databases met the eligibility criteria. Regarding the cell characteristics used in the selected studies, most used MSCs from different human sources, characterized before administration, using a lower dose than HSCs, but by similar routes. HSCs were from human umbilical cord blood or animal BM and the recipients were mainly irradiated immunodeficient mouse. The cotransplantation was evaluated mainly by chimerism followed by hematopoietic reconstruction, showing HSC engraft improvement with concomitant MSC implantation.
Research conclusions
The preclinical findings in this systematic review validate the potential of MSCs to enable HSC engraftment in vivo in both xenogeneic and allogeneic hematopoietic cell transplantation animal models.
Research perspectives
The use of HSCs in BMT shows promise for improvement of engraftment in animal models; however, there is still a need for MSC standardization to evaluate the real potential of the therapy in humans.
