Choudhary RK, Choudhary S, Tripathi A. Emergence of the stromal vascular fraction and secretome in regenerative medicine. World J Stem Cells 2024; 16(10): 896-899 [PMID: 39493826 DOI: 10.4252/wjsc.v16.i10.896]
Corresponding Author of This Article
Ratan Kumar Choudhary, PhD, Assistant Professor, College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, NH 95, Firozpur Road, Ludhiana 141004, Punjab, India. vetdrrkc@gmail.com
Research Domain of This Article
Research & Experimental Medicine
Article-Type of This Article
Letter to the Editor
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Ratan Kumar Choudhary, Shanti Choudhary, College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India
Abhishek Tripathi, Hilltop Animal Hospital, Palatine, IL 60074, United States
Author contributions: Choudhary RK and Tripathi A conceived and drafted the editorial; Choudhary S provided critical revisions to the manuscript and was helpful in canine stromal vascular fraction work; All authors read and approved the final version of the manuscript.
Supported bythe Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi, No. BT/PR42179/AAQ/1/814/2021; and SERB-State University Research Excellence, No. SUR/2022/001952.
Conflict-of-interest statement: All authors report no relevant conflicts of interest for this article.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Ratan Kumar Choudhary, PhD, Assistant Professor, College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, NH 95, Firozpur Road, Ludhiana 141004, Punjab, India. vetdrrkc@gmail.com
Received: August 8, 2024 Revised: September 24, 2024 Accepted: September 29, 2024 Published online: October 26, 2024 Processing time: 77 Days and 20.3 Hours
Abstract
Recently, we read a mini-review published by Jeyaraman et al. The article explored the optimal methods for isolating mesenchymal stromal cells from adipose tissue-derived stromal vascular fraction (SVF). Key factors include tissue source, processing techniques, cell viability assessment, and the advantages/disadvantages of autologous vs allogeneic use. The authors emphasized the need for standardized protocols for SVF isolation, ethical and regulatory standards for cell-based therapy, and safety to advance mesenchymal stromal cell-based therapies in human patients. This manuscript shares our perspective on SVF isolation in canines. We discussed future directions to potentiate effective regenerative medicine therapeutics in human and veterinary medicine.
Core Tip: A recent mini-review highlighted critical steps for optimal stromal vascular fraction (SVF) isolation from adipose tissue, including liposuction refinement, tissue handling, enzymatic digestion, and rigorous quality control for cell viability and purity. This article expanded upon the review by examining the advantages and limitations of SVF isolation, exploring SVF isolation in canine patients, and discussing the future potential of SVF in regenerative medicine.
