Published online Jan 26, 2021. doi: 10.4252/wjsc.v13.i1.1
Peer-review started: June 26, 2020
First decision: September 18, 2020
Revised: September 28, 2020
Accepted: November 9, 2020
Article in press: November 9, 2020
Published online: January 26, 2021
The recent progress in derivation of pluripotent stem cells (PSCs) from farm animals opens new approaches not only for reproduction, genetic engineering, treatment and conservation of these species, but also for screening novel drugs for their efficacy and toxicity, and modelling of human diseases. Initial attempts to derive PSCs from the inner cell mass of blastocyst stages in farm animals were largely unsuccessful as either the cells survived for only a few passages, or lost their cellular potency; indicating that the protocols which allowed the derivation of murine or human embryonic stem (ES) cells were not sufficient to support the maintenance of ES cells from farm animals. This scenario changed by the innovation of induced pluripotency and by the development of the 3 inhibitor culture conditions to support naïve pluripotency in ES cells from livestock species. However, the long-term culture of livestock PSCs while maintaining the full pluripotency is still challenging, and requires further refinements. Here, we review the current achievements in the derivation of PSCs from farm animals, and discuss the potential application areas.
Core Tip: The successful derivation of pluripotent stem cells (PSCs) from livestock represents an ideal model for the progress of veterinary, biomedical and regenerative medicine. The inherent properties of self-renewal and differentiation make PSCs an ideal raw biomaterial for innovative approaches in artificial reproductive techniques, cell-based therapy, disease modelling, drug testing, organ generation, breed conservation and in vitro meat production. In this review, we present the current status of PSCs application for the development of livestock farming and their potential applications for human welfare.