Metabolic shift in liver: Correlation between perfusion temperature and hypoxia inducible factor-1α

Figure 1 Liver oxygen uptake rate and lactate dehydrogenase release rate in rat livers perfused for 6. A: OUR; and B: Lactate-dehydrogenase (LDH) release rate over 6 h of perfusion at 10, 20, 30 or 37 °C (n = 6/group; ^bP < 0.01 vs 20 °C). OUR: Oxygen uptake rate.

Figure 2 Bile flow, ATP and glycogen levels in rat livers perfused for 6 h. A: Basal bile flow; B: Bile flow during perfusion; C: ATP content; and D: Glycogen content in livers perfused for 6 h at 10, 20, 30 or 37 °C (n = 6/group; B: ^bP < 0.01 vs 10 and 20 °C; C: ^bP < 0.01 vs 30 and 37 °C, ^dP < 0.01 vs 20 °C; D: ^fP < 0.01 vs 37 °C).

Figure 3 pH levels and lactate release in rat livers perfused for 6 h. A: Normalized pH; B: Lactic acid release in livers perfused for 6 h at 10, 20, 30 or 37 °C (n = 6/group; ^bP < 0.01 vs 10 and 20 °C; ^cP < 0.05 vs 10 and 20 °C).

Figure 4 Hepatic hypoxia inducible factor-1α expression in rat livers perfused for 6 h. A: mRNA; and B: Protein expression of hypoxia inducible factor (HIF)-1α in livers perfused for 6 h at 10, 20, 30 or 37 °C (n = 6/group; ^aP < 0.05, ^bP < 0.01 vs 10 and 20 °C; ^cP < 0.05 vs 10 and 20 °C).