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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 28, 2015; 21(4): 1108-1116
Published online Jan 28, 2015. doi: 10.3748/wjg.v21.i4.1108
Published online Jan 28, 2015. doi: 10.3748/wjg.v21.i4.1108
Metabolic shift in liver: Correlation between perfusion temperature and hypoxia inducible factor-1α
Andrea Ferrigno, Laura Giuseppina Di Pasqua, Alberto Bianchi, Plinio Richelmi, Mariapia Vairetti, Unit of Cellular and Molecular Pharmacology and Toxicology, Department of Internal Medicine and Therapeutics, University of Pavia, Pavia 27100, Italy
Author contributions: Ferrigno A, Vairetti M and Richelmi P conceived of the study, analyzed and interpreted the data, and drafted the manuscript; Di Pasqua LG and Bianchi A acquire the data; all authors revised and approved the final version of the manuscript.
Supported by Grant from Fondazione Cariplo, No. 2011-0439.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Andrea Ferrigno, PhD, Unit of Cellular and Molecular Pharmacology and Toxicology, Department of Internal Medicine and Therapeutics, University of Pavia, via Ferrata 9/A, Pavia 27100, Italy. andrea.ferrigno@unipv.it
Telephone: +39-3-82986451 Fax: +39-3-82986347
Received: June 20, 2014
Peer-review started: June 20, 2014
First decision: July 21, 2014
Revised: August 1, 2014
Accepted: September 29, 2014
Article in press: September 30, 2014
Published online: January 28, 2015
Peer-review started: June 20, 2014
First decision: July 21, 2014
Revised: August 1, 2014
Accepted: September 29, 2014
Article in press: September 30, 2014
Published online: January 28, 2015
Core Tip
Core tip: Among the techniques developed to improve the preservation of marginal organs for transplantation, hypothermic perfusion is the preferred choice. We show that it is possible to perfuse a rat liver at 20 °C without incurring ischemia. We evaluated liver injury, energetic status, lactate release, and hypoxia inducible factor-1α expression. Results show that symptoms of ischemia appear at temperatures > 20 °C, whereas there is no detectable advantage below 20 °C. These findings have interesting implications in liver preservation; maintaining the liver in a mild metabolic state could be useful for pharmacologic treatment and regeneration of the energetic status in ATP-depleted organs.