Randomized Controlled Trial
Copyright ©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 21, 2015; 21(7): 2183-2190
Published online Feb 21, 2015. doi: 10.3748/wjg.v21.i7.2183
MicroRNA profiling of the intestine during hypothermic circulatory arrest in swine
Wei-Bin Lin, Meng-Ya Liang, Guang-Xian Chen, Xiao Yang, Han Qin, Jian-Ping Yao, Kang-Ni Feng, Zhong-Kai Wu
Wei-Bin Lin, Meng-Ya Liang, Guang-Xian Chen, Xiao Yang, Han Qin, Jian-Ping Yao, Kang-Ni Feng, Zhong-Kai Wu, 2nd Department of Cardiac Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China
Author contributions: Lin WB conducted the molecular studies, analyzed the data and drafted the manuscript; Chen GX, Liang MY and Yao JP participated in establishing the cardiopulmonary bypass model; Feng KN and Qin H participated in the design of the study and performed the statistical analysis; Wu ZK and Yang X conceived the study, participated in its design and coordination and helped to draft the manuscript; all authors read and approved the final manuscript.
Supported by National Basic Research Program of China (973 Program), No. 2010CB5295007; Pearl River Scholar Program, No. 80000-3210003; and Natural Science Fund of China, No. 81370215.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Zhong-Kai Wu, MD, PhD, 2nd Department of Cardiac surgery, the First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan II Road, Guangzhou 510080, Guangdong Province, China. wuzhk@mail.sysu.edu.cn
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Received: May 31, 2014
Peer-review started: June 1, 2014
First decision: July 21, 2014
Revised: August 12, 2014
Accepted: September 5, 2014
Article in press: September 5, 2014
Published online: February 21, 2015
Processing time: 255 Days and 21.8 Hours
Core Tip

Core tip: Swine intestine was subjected to hypothermia, cardiopulmonary bypass and ischemia/reperfusion following hypothermic circulatory arrest (HCA). These factors caused barrier dysfunction, resulting in gastrointestinal complications. Histopathological and microRNA (miRNA) array analyses were used to investigate the effects of HCA on the gut barrier. HCA was found to disturb barrier function in the small intestine and influence the miRNA levels in swine. Our results contribute to the body of research examining gut barrier function following HCA in vivo.