Shan KS, Li WW, Ren W, Kong S, Peng LP, Zhuo HQ, Tian SB. LncRNA cancer susceptibility 20 regulates the metastasis of human gastric cancer cells via the miR-143-5p/MEMO1 molecular axis. World J Gastroenterol 2022; 28(16): 1656-1670 [PMID: 35581960 DOI: 10.3748/wjg.v28.i16.1656]
Corresponding Author of This Article
Shu-Bo Tian, PhD, Chief Doctor, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China. ttkl_bo@126.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Ke-Shu Shan, Shuai Kong, Li-Pan Peng, Hong-Qing Zhuo, Shu-Bo Tian, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong Province, China
Ke-Shu Shan, Shuai Kong, Li-Pan Peng, Hong-Qing Zhuo, Shu-Bo Tian, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China
Wei-Wei Li, Department of Critical Care Medicine, The 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250031, Shandong Province, China
Wang Ren, Department of General Surgery, People's Hospital of Sishui County, Jining 273200, Shandong Province, China
Author contributions: Shan KS and Li WW contributed equally to this work; Tian SB and Shan KS designed the study; Shan KS, Li WW and Ren W performed all experiments; Kong S and Peng LP collected tissue samples and the clinical data; Shan KS and Li WW analyzed and interpreted the data; Tian SB and Zhuo HQ drafted the manuscript; all authors read and approved the final manuscript.
Supported byShandong Province Medicine and Health Science and Technology Development Plan Project, No. 2019WS477.
Institutional review board statement: The study was reviewed and approved by the Shandong Provincial Hospital Institutional Review Board, No. SZRJJ: NO. 2020.
Conflict-of-interest statement: The authors declare no conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Shu-Bo Tian, PhD, Chief Doctor, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China. ttkl_bo@126.com
Received: October 19, 2021 Peer-review started: October 19, 2021 First decision: December 12, 2021 Revised: December 26, 2021 Accepted: March 25, 2022 Article in press: March 25, 2022 Published online: April 28, 2022 Processing time: 186 Days and 15.9 Hours
ARTICLE HIGHLIGHTS
Research background
LncRNAs have been indicated to play critical roles in gastric cancer (GC) tumorigenesis and progression. However, their roles in GC remain to be further elucidated.
Research motivation
To discover promising diagnostic and therapeutic targets for GC.
Research objectives
To investigate the underlying mechanisms of the lncRNA cancer susceptibility 20 (CASC20) in GC.
Research methods
The expression of CASC20, miR-143-5p and MEMO1 genes were detected by quantitative real-time polymerase chain reaction. The oncogenic functions of CASC20 was investigated by experiments including cell counting kit-8, colony formation, wound-healing and Transwell assays in GC cells in vitro. Bioinformatics and dual-luciferase report assay were performed for determining the regulatory relationship of lncRNA-miRNA-mRNA.
Research results
We found that the expression of lncRNA CASC20 was significantly upregulated in GC tissues and cell lines, and increased expression of CASC20 was positively correlated with lymph node metastasis in patients with GC. Additionally, knockdown of CASC20 could inhibit GC cells growth, migration and invasion, whereas its overexpression could reverse these effects. Mechanistically, CASC20 specifically inhibited miR-143-5p expression, thus up-regulating MEMO1 expression.
Research conclusions
CASC20 promotes GC cell metastasis via adsorbing miR-143-5p and up-regulating MEMO1, it plays an important oncogenic function in GC.
Research perspectives
This study identified the lncRNA CASC20/miR-143-5p/MEMO1 axis in GC and provided insights into the mechanistic relationships among CASC20, miR-143-5p and MEMO. The results of our research suggest that CASC20 may act as a promising diagnostic marker for GC.
