Shan KS, Li WW, Ren W, Kong S, Peng LP, Zhuo HQ, Tian SB. LncRNA cancer susceptibility 20 regulates the metastasis of human gastric cancer cells via the miR-143-5p/MEMO1 molecular axis. World J Gastroenterol 2022; 28(16): 1656-1670 [PMID: 35581960 DOI: 10.3748/wjg.v28.i16.1656]
Corresponding Author of This Article
Shu-Bo Tian, PhD, Chief Doctor, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China. ttkl_bo@126.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Ke-Shu Shan, Shuai Kong, Li-Pan Peng, Hong-Qing Zhuo, Shu-Bo Tian, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong Province, China
Ke-Shu Shan, Shuai Kong, Li-Pan Peng, Hong-Qing Zhuo, Shu-Bo Tian, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China
Wei-Wei Li, Department of Critical Care Medicine, The 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250031, Shandong Province, China
Wang Ren, Department of General Surgery, People's Hospital of Sishui County, Jining 273200, Shandong Province, China
Author contributions: Shan KS and Li WW contributed equally to this work; Tian SB and Shan KS designed the study; Shan KS, Li WW and Ren W performed all experiments; Kong S and Peng LP collected tissue samples and the clinical data; Shan KS and Li WW analyzed and interpreted the data; Tian SB and Zhuo HQ drafted the manuscript; all authors read and approved the final manuscript.
Supported byShandong Province Medicine and Health Science and Technology Development Plan Project, No. 2019WS477.
Institutional review board statement: The study was reviewed and approved by the Shandong Provincial Hospital Institutional Review Board, No. SZRJJ: NO. 2020.
Conflict-of-interest statement: The authors declare no conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Shu-Bo Tian, PhD, Chief Doctor, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China. ttkl_bo@126.com
Received: October 19, 2021 Peer-review started: October 19, 2021 First decision: December 12, 2021 Revised: December 26, 2021 Accepted: March 25, 2022 Article in press: March 25, 2022 Published online: April 28, 2022 Processing time: 186 Days and 15.9 Hours
Abstract
BACKGROUND
Gastric cancer (GC) is considered as one of the most widespread malignancies. Emerging evidence has shown that lncRNAs can function as important oncogenes or tumor suppressors during GC progression.
AIM
To investigate the effect and mechanism of lncRNA cancer susceptibility 20 (CASC20) in the proliferation and metastasis of GC cells.
METHODS
Data mining and clinical samples were used to evaluate the expression of CASC20 in GC and adjacent tissues. CASC20 was down-regulated in GC cells by short-interfering RNA. Cell proliferation was evaluated by CCK-8 assay, and cell migration and invasion were detected by wound healing and Transwell assays. The expressions of proteins related to epithelial-mesenchymal transition were detected by western blot assay.
RESULTS
The expression of CASC20 was increased in GC tumor tissues and various GC cell lines. High CASC20 expression was correlated with a high risk of lymphatic metastasis and poor prognosis in GC patients. In vitro assays showed that silencing CASC20 reduced cell proliferation, migration, and invasion in GC cells. Mechanistic studies revealed that CASC20 exhibits oncogenic functions by regulating MEMO1 expression through competitive endogenous binding to miR-143-5p, leading to induction of epithelial-mesenchymal transition.
CONCLUSION
Our findings indicate that CASC20 serves as a tumor promoter by regulating metastasis in GC via the miR-143-5p/MEMO1 axis. CASC20 may be a potential therapeutic target for GC.
Core Tip: LncRNA cancer susceptibility 20 (CASC20) is upregulated in gastric cancer (GC) and associated with a higher risk of lymphatic metastasis and poor prognosis of patients. CASC20 can promote the proliferation, invasion and metastasis of GC cells by absorbing miR-143-5p to upregulate MEMO1, thereby induced epithelial-mesenchymal transition in vitro.
