Published online Mar 21, 2015. doi: 10.3748/wjg.v21.i11.3245
Revised: October 11, 2014
Accepted: December 14, 2014
Published online: March 21, 2015
Processing time: 223 Days and 2.5 Hours
AIM
To investigate the biological role of miR-1290 in esophageal squamous cell carcinoma (ESCC) progression and invasion and the underlying mechanism.
METHODS
Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate miR-1290 expression in ESCC tissue samples. The roles of miR-1290 in cell proliferation, migration and invasion were identified using miR-1290 mimic-transfected cells. In addition, the regulatory effect of miR-1290 on suppressor of cancer cell invasion (SCAI) was evaluated using qRT-PCR, Western blot analysis and a dual luciferase reporter assay.
RESULTS
miR-1290 was significantly upregulated in ESCC tissue samples compared with normal adjacent tissues (9.213 ± 1.150
CONCLUSION
Our findings suggested that miR-1290 may play an oncogenic role in cellular processes of ESCC.
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Core Tip: In this study, we reported the clinical significance and biological effects of miR-1290 in esophageal squamous cell carcinoma (ESCC). We found that miR-1290 was significantly up-regulated in ESCC tissues. Moreover, we showed that ectopic expression of miR-1290 significantly promoted ESCC cell growth, migration and invasion. Further investigation revealed that suppressor of cancer cell invasion was a downstream target of miR-1290.