Circulating immune parameters-based nomogram for predicting malignancy in laryngeal neoplasm

Table 1 Clinical immune cell data of patients in the primary cohort

Variables	Laryngeal benign lesion	Laryngeal premalignant lesion	Laryngeal malignant lesion	P value
Age, yr	50.58 ± 8.66	59.91 ± 9.36	61.67 ± 9.58	< 0.001
Gender, male, n (%)	37 (74.0%)	52 (96.3%)	52 (100%)	< 0.001
Smokers, n (%)	14 (28.0%)	18 (33.3%)	29 (55.8%)	0.009
Smoke index	123.66 ± 245.44	235.74 ± 424.28	484.31 ± 447.77	< 0.001
Drinkers, n (%)	6 (12.0%)	9 (16.7%)	14 (26.9%)	0.140
Drinker index	1.40 ± 4.85	2.43 ± 7.21	4.37 ± 8.38	0.096
Lymphocytes, %	33.30 ± 9.02	29.89 ± 9.37	29.66 ± 8.57	0.077
Monocytes, %	6.86 ± 3.47	6.73 ± 1.41	7.80 ± 2.36	0.064
Neutrophils, %	57.86 ± 9.61	60.25 ± 11.08	59.95 ± 8.82	0.414
NLR	1.98 ± 1.02	2.43 ± 1.49	2.31 ± 1.09	0.158
LMR	5.29 ± 1.82	4.50 ± 1.33	4.24 ± 2.26	0.012
CD3+ T cells, %	68.48 ± 8.41	62.12 ± 12.00	61.82 ± 8.93	0.001
CD8+ T cells, %	25.77 ± 7.83	23.64 ± 7.82	23.30 ± 7.18	0.210
CD4+ T cells, %	39.13 ± 7.16	35.09 ± 8.21	36.11 ± 9.27	0.039
NK cells, %	17.35 ± 8.71	25.40 ± 12.65	24.80 ± 9.21	< 0.001
B cells, %	13.46 ± 4.14	11.51 ± 4.29	12.31 + 6.63	0.157
CD4/CD8+ T cell ratio	1.71 ± 0.72	1.64 + 0.63	1.78 + 0.94	0.629
NK/CD3+ T cell ratio	0.27 ± 0.20	0.47 ± 0.45	0.44 ± 0.22	0.003
NK/CD4+ T cell ratio	0.46 ± 0.29	0.85 ± 0.78	0.79 ± 0.42	0.001
NK/CD8+ T cell ratio	0.75 ± 0.73	1.28 ± 1.35	1.23 ± 0.74	0.014

LMR: Lymphocyte to monocyte ratio; NK cell: Natural killer cell; NLR: Neutrophil to lymphocyte ratio.

Table 2 Circulating immune features for prediction of laryngeal malignant lesion

Intercept and variables	β1	Odds ratio (95%CI)	P value
Intercept	-5.500		< 0.0001
Monocytes, %	1.235	2.351 (1.258-10.066)	0.019
CD8+ T cells, %	2.154	3.303 (2.562-33.856)	< 0.001
CD4+ T cells, %	2.701	2.730 (2.422-123.774)	< 0.001
B cells, %	1.464	2.315 (1.320-16.195)	0.021
CD4/CD8+ T cell ratio	2.425	2.004 (1.413-247.045)	0.045

¹ is the regression coefficient. Circulating immune features selected from least absolute shrinkage and selection operator method was entered into the multivariable logistic regression analysis. CI: Confidence interval.