Overexpression of AMPD2 indicates poor prognosis in colorectal cancer patients via the Notch3 signaling pathway

Table 1 Correlations between clinicopathological characteristics and both AMPD2 and Notch3 expression in 201 colorectal cancer patients

Parameters	Total (n = 201)	AMPD2 expression		Chi-square value	P value
		High	Low
Gender				0.664	0.415
Male	114	32	82
Female	87	20	67
Age (yr)				0.004	0.953
< 60	78	20	58
≥ 60	123	32	91
Location				3.045	0.081
Colon	80	26	54
Rectum	121	26	95
Vascular invasion				0.502	0.479
Negative	165	41	124
Positive	36	11	25
Neural invasion				0.780	0.337
Negative	170	42	128
Positive	31	10	21
Depth of tumor				5.270	0.022a
T1/T2	60	9	51
T3/T4	141	43	98
Lymph metastasis				0.048	0.826
N0	94	25	69
N1/N2	107	27	80
Differentiation				3.887	0.037a
Well/mod	184	44	140
Poor	17	8	9
TNM stage				0.487	0.485
I-II	96	27	69
III-IV	105	25	80
Notch3				0.389	0.553
High	140	38	102
Low	61	14	47

^aP < 0.05 was considered statistically significant.

Table 2 Cox proportional model predicts the relationship between AMPD2, Notch3 and clinicopathological parameters and survival

Clinicopathological Parameters	Univariate		Multivariate
	HR (95%CI)	P value	HR (95%CI)	P value
Gender (female/male)	1.004 (0.636-1.585)	0.986	0.973 (0.610-1.552)	0.909
Age (≥ 60/< 60)	1.060 (0.664-1.693)	0.806	1.066 (0.661-1.721)	0.793
Location (rectum/colon)	0.889 (0.561-1.409)	0.616	0.888 (0.553-1.428)	0.625
Differentiation (poor, moderate/well)	0.496 (0.249-0.988)	0.041	0.727 (0.450-1.173)	0.191
TNM stage (III-IV/I-II)	3.198 (1.915-5.343)	< 0.001a	3.727 (2.206-6.296)	<0.001a
AMPD2 (high/low)	1.769 (1.094-2.862)	0.020a	2.133 (1.295-3.513)	0.003a
Notch3 (high/low)	1.237 (0.747-2.048)	0.408	1.119 (0.668-1.874)	0.669

^aP < 0.05 was considered statistically significant. HR: Hazard ratio; CI: Confidence interval.