MiRNA-200a and miRNA-200b expression, and vitamin-D level: Prognostic significance in obese non-diabetic and obese type 2 diabetes mellitus individuals

Table 1 Comparison of biochemical parameters in healthy controls, obese, and obese with type 2 diabetes mellitus participants, mean ± SD

Biochemical parameters	Healthy controls	Obese	Obese type 2 diabetes	P value
Fasting blood glucose (mg/dL)	82.91 ± 8.34	87.48 ± 5.50	185.4 ± 39.50	< 0.0001
Postprandial blood glucose (mg/dL)	128.3 ± 8.44	135.0 ± 5.54	239.7 ± 43.21	< 0.0001
HbA1c (%)	5.18 ± 4.47	5.65 ± 0.54	8.28 ± 0.55	< 0.0001
HDL (mg/dL)	56.14 ± 5.90	45.91 ± 13.49	43.32 ± 11.66	< 0.0001
LDL (mg/dL)	98.35 ± 10.89	203.6 ± 18.65	202.3 ± 13.71	< 0.0001
TG (mg/dL)	124.7 ± 15.27	282.3 ± 76.87	424.7 ± 76.32	< 0.0001
Cholesterol (mg/dL)	161.9 ± 23.80	237.7 ± 34.73	279.7 ± 27.58	< 0.0001
VLDL (mg/dL)	25.27 ± 2.40	34.89 ± 5.68	35.50 ± 4.79	< 0.0001

HDL: High-density lipoprotein; LDL: Low-density lipoprotein; TG: Triglycerides; VLDL: Very low-density lipoprotein.

Table 2 Correlation analysis of miRNA-200a and miRNA200b with biochemical parameters

Biochemical parameters	miRNA-200a		miRNA-200b
	r	P value	r	P value
Fasting blood glucose	0.16	0.09	0.26	0.005
Postprandial blood glucose	0.25	0.007	0.28	0.003
HbA1c	0.21	0.02	0.23	0.01
HDL	0.01	0.88	-0.27	0.004
LDL	0.14	0.13	0.03	0.73
TG	0.11	0.24	0.31	0.001
Cholesterol	0.24	0.009	0.34	< 0.0001
VLDL	0.08	0.40	0.26	0.006

HDL: High-density lipoprotein; LDL: Low-density lipoprotein; TG: Triglycerides; VLDL: Very low-density lipoprotein.

Table 3 Area under the curve for miRNA-200a, miRNA-200b, sensitivity, specificity and cutoff value

Variables	AUC	95%CI	Sensitivity	Specificity	Cutoff	P value
miRNA-200a	0.64	0.53-0.75	75%	57%	2.07 fold	0.02
miRNA-200b	0.75	0.64-0.75	75%	71%	4.12 fold	< 0.0001

AUC: Area under the curve.