Published online Dec 26, 2024. doi: 10.12998/wjcc.v12.i36.6916
Revised: September 18, 2024
Accepted: October 9, 2024
Published online: December 26, 2024
Processing time: 140 Days and 0.5 Hours
Obesity and type 2 diabetes mellitus (T2DM) are frequent co-occurring disorders that affect regular metabolic functions. Obesity has also been linked to an inc
To evaluate miRNA-200a and miRNA-200b expression, and vitamin-D levels in obese and obese T2DM individuals.
This study included 210 participants, of which, 82 were obese (BMI > 30 kg/m2) without T2DM, 28 were obese with T2DM, and 100 were healthy controls. BMI was evaluated and both fasting and postprandial blood glucose were used to confirm T2DM. Exosomal miRNA-200a and miRNA-200b expression were analyzed using real-time PCR using Taqman probes, and vitamin-D levels were evaluated using an electrochemiluminescence-based immunoassay technique. All data analyses were performed using SPSS 20.0 and GraphPad Prism 5 software.
Overall, a 2.20- and 4.40-fold increase in miRNA-200a and miRNA-200b expression was observed among participants compared to healthy controls. MiRNA-200a and miRNA-200b expression among obese participants increased 2.40-fold and 3.93-fold, respectively, while in obese T2DM participants these values were 2.67-fold, and 5.78-fold, respectively, and these differences were found to be statistically significant (P = 0.02) (P < 0.0001). Obese participants showed a vitamin D level of 34.27 ng/mL, while in obese-T2DM participants vitamin D level was 22.21 ng/mL (P < 0.0001). Vitamin D was negatively correlated with miRNA-200a (r = -0.22, P = 0.01) and miRNA-200b (r = -0.19, P = 0.04). MiRNA-200a sensitivity was 75%, and specificity was 57%, with a cutoff value of 2.07-fold. MiRNA-200b sensitivity was 75%, and specificity was 71% with a cutoff value of 4.12-fold, suggesting that miRNA-200a and miRNA-200b with an increased expression of 2.07- and 4.12-fold could be predictive indicators for the risk of diabetes in obese participants.
MiRNA-200a and miRNA-200b were higher in diabetic obese participants vs non-diabetic obese participants, and insufficient vitamin D levels in obese T2DM participants may be involved in poor clinical outcome.
Core Tip: Obesity and being overweight are widespread health issues in modern societies, and their growing prevalence poses a serious public health challenge due to both the financial burden and health risks involved. In humans, the expression of miRNAs in adipose tissue has been demonstrated to be associated with various metabolic factors, including body mass index, adipogenesis, blood sugar levels, and leptin concentrations. The microRNA (miRNA)-200 family is reported to be more prevalent in human β-cells than in α-cells. The expression of miRNA-200a and miRNA-200b is regulated by thioredoxin-interacting protein, a proapoptotic regulator. It has been suggested that a deficiency in vitamin D is closely associated with obesity and plays a role in the development of insulin resistance and type 2 diabetes mellitus (T2DM). Studies have indicated that vitamin D influences the expression of miRNAs both in adipocytes in laboratory settings and in adipose tissue in living organisms. Therefore, the current work examines miRNA-200a and miRNA-200b expression, and vitamin-D level in obese non-diabetic and obese T2DM individuals.