Correlation analysis of human papillomavirus E6/E7 mRNA detection with diagnosis, prognosis and recurrence risk in patients with cervical epithelioma

Table 1 Comparison of the positive expression rates of human papillomavirus-DNA and human papillomavirus E6/E7 mRNA in each group (%)

Group	Example number	HPV E6/E7 mRNA		HPV (PCR-reverse point hybridization)
		Positive	Negative	Positive	Negative
CINI level	48	13 (27.1)	35 (72.9)	15 (31.3)	33 (68.7)
CIN II level	31	24 (77.4)	7 (22.6)	21 (67.7)	10 (32.3)
CIN III level	16	12 (75.0)	4 (25.0)	11 (68.7)	5 (31.3)
Squamous or adenocarcinoma	5	3 (18.8)	2 (12.5)	4 (25.0)	1 (6.3)
χ2		23.483		14.669
P value		< 0.001		0.002

CIN: Cervical intraepithelial neoplasia; HPV: Human papilloma virus; PCR: Polymerase chain reaction.

Table 2 Results of the univariate analysis affecting the diagnosis of cervical intraepithelial neoplasia high-grade lesions (%)

Project	CIN grade (n = 52)	CIN grade I (n = 48)	χ2	P value
Age			0.495	0.482
≥ 45	15 (28.8)	17 (35.4)
< 45	37 (71.2)	31 (64.6)
Gravidity			0.031	0.861
≥ 2	16 (30.8)	14 (29.2)
< 2	36 (69.2)	34 (70.8)
Parity			0.495	0.482
≥ 2	15 (28.8)	17 (35.4)
< 2	37 (71.2)	31 (64.6)
HPV (PCR reverse-dot hybridization)			14.408	< 0.001
Positive	36 (69.2)	15 (31.3)
Negative	16 (30.8)	33 (68.7)
HPV E6/E7 mRNA			22.960	< 0.001
Positive	39 (75.0)	13 (27.1)
Negative	13 (25.0)	35 (72.9)

CIN: Cervical intraepithelial neoplasia; HPV: Human papilloma virus; PCR: Polymerase chain reaction.

Table 3 Results of logistic factor analysis

Metric	β	SE	Walds	P value	OR	95%CI
HPV (PCR reverse-dot hybridization)	-0.163	0.511	0.102	0.007	1.850	1.312-2.312
HPV E6/E7 mRNA	0.630	0.510	0.523	0.022	1.877	1.690-5.120

HPV: Human papilloma virus; PCR: Polymerase chain reaction; OR: Odds ratio; CI: Confidence interval.

Table 4 Effect of positive human papilloma virus E6/E7 mRNA expression on the development of disease in patients with cervical intraepithelial neoplasia grade I (%)

HPV E6/E7 mRNA	Example number	CIN I level	CIN II level
Positive	13	4 (30.8)	9 (69.2)
Negative	35	25 (71.4)	10 (28.6)
χ2		6.553
P value		0.010

CIN: Cervical intraepithelial neoplasia; HPV: Human papilloma virus.