Encouraging specific biomarkers-based therapeutic strategies for hepatocellular carcinoma

Table 1 Amino acid peptide sequences and immunogenicity of alpha-fetoprotein epitopes

No.	Starting	Numbers	Fragment	Immunogenicity
1	7	9	IFLIFLLNF	Sub-immunodominant Ag
2	137	9	PLFQVPEPV	Immunodominant Ag
3	150	9	AYEEDRETF	Sub-immunodominant Ag
4	158	9	FMNKFIYEI	Immunodominant Ag
5	218	9	LLNQHACAV	Sub-immunodominant Ag
6	235	9	FQAITVTKL	Sub-immunodominant Ag
7	249	10	KVNFTEIQKL	Immunodominant Ag
8	307	9	TTLERGQCII	Sub-immunodominant Ag
9	321	9	KPEGLSPNL	Immunodominant Ag
10	325	10	GLSPNLNRFL	Immunodominant Ag
11	357	9	EYSRRHPQL	Immunodominant Ag
12	364	10	QLAVSVILRV	Immunodominant Ag
13	403	9	KYIQESQAL	Immunodominant Ag
14	414	9	RSCGLFQKL	Immunodominant Ag
15	424	9	EYYLQNAFL	Immunodominant Ag
16	434	9	AYTKKAPQL	Immunodominant Ag
17	485	10	CIRHEMTPV	Sub-immunodominant Ag
18	492	9	PVNPGVGQC	Sub-immunodominant Ag
19	503	9	SYANRRPCF	Sub-immunodominant Ag
20	507	10	NRRPCFSSLV	Sub-immunodominant Ag
21	542	9	GVALQTMKQ	Immunodominant Ag
22	547	10	TMKQEFLINL	Sub-immunodominant Ag
23	555	9	NLVKQKPQI	Sub-immunodominant Ag
24	591	9	CFAEEGQKL	Sub-immunodominant Ag

Ag: Antigen; Fragment: Fragment of alpha-fetoprotein (AFP) peptide chain; Numbers: Amino acid numbers of AFP peptide chain; Starting: Starting point of AFP peptide chain.

Table 2 Glypican-3 as molecule-target for hepatocellular carcinoma immunotherapy

Group	Name	Species	Epitopes	Verifying/applying
Antibody	M18D04/19B11	Mouse	N-terminal (aa: 25-358)	Basic studies
	A1836A	Mouse	N- terminal	Basic studies
	GPC3-C02	Mouse	C- terminal	Basic studies
	GC33	Mouse	C-terminal (aa: 524-563)	Preclinical trial studies
	hGC33	Human	C- terminal (aa: 524-563)	Clinical trial-II
	HS20	Human	Heparan sulfate chain	Preclinical trial
	sGPC3	Human	—	Preclinical trial
Vaccines	GPC3298-306	Mouse	298-306 peptide	Clinical trial-II
	GPC3144-152	Mouse	144-152 peptide	Clinical trial-II
miRNA	miR-219-5p	Human	—	In vitro or in vivo studies
	miR-520c-3p	Human	—	In vitro studies
	miR-1271	Human	—	In vitro studies
shRNA	GPC3 shRNA	Human	—	In vitro or in vivo studies
siRNA	GPC3 siRNA	Human	—	In vitro or in vivo studies

GPC-3: Glypican-3; aa: Amino acid; miR: MicroRNA; shRNA: Small hairpin RNA; siRNA: Small interfering RNA.