Treatment of pediatric intracranial dissecting aneurysm with clipping and angioplasty, and next-generation sequencing analysis: A case report and literature review

Abstract

BACKGROUND

Large intracranial dissecting aneurysm (IDA) in the anterior cerebral circulation is rare in children. There has been no consensus on the diagnosis and treatment for IDA in children.

CASE SUMMARY

We report a 3-year-old boy with a large ruptured IDA in the right middle cerebral artery (16 mm × 14 mm). The IDA was successfully managed with clipping and angioplasty. Next-generation sequencing of the blood sample followed by bioinformatics analysis suggested that the rs78977446 variant of the ADAMTS13 gene is a risk for pediatric IDA. Three years after surgery, the boy was develop-mentally normal.

CONCLUSION

Clipping and angioplasty are effective treatments for ruptured IDA in the anterior cerebral circulation. ADAMTS13 rs78977446 is a risk factor for pediatric IDA.

Key Words: Intracranial dissecting aneurysm, Clipping, Pathogenic variants, ADAMTS13, Case report

Core Tip: The index case was a 3-year-old boy with a large ruptured intracranial dissecting aneurysm in the right middle cerebral artery (16 mm × 14 mm). He was successfully treated by clipping and angioplasty. Whole-genome high-throughput sequencing identified the rs78977446 variant of the ADAMTS13 gene. Bioinformatics analysis using the American College of Medical Genetics guidelines and literature search suggested that this variant is a risk factor for pediatric intracranial dissecting aneurysm.

INTRODUCTION

Rupture of intracranial dissecting aneurysms (IDA) is a cause of subarachnoid hemorrhage (SAH) in children[1]. The incidence of IDA is estimated to be no more than that for cervical dissecting artery (2.6–3.0 per 100000 people per year)[2,3]. Both genetic and environmental factors contribute to the development of pediatric IDA[4]. At the level of pathology, ultimate formation of intramural hematoma between the intima and media consists of tear of artery and rupture of vasa vasorum[5]. IDA is associated with syphilis[6], connective tissue diseases[7], atherosclerosis[8], infection[9], migraine[10], hyperhomocysteinemia[10], and alpha-1 antitrypsin deficiency[11]. A key event in dissec-ting aneurysms is the sudden widespread disruption of the internal elastic lamina and media[12,13].

IDA in children, and particularly in the anterior cerebral circulation, has rarely been reported and represents a formidable challenge in both the diagnosis and treatment[14].

We report a case of SAH caused by ruptured IDA in the anterior cerebral circul-ation. The patient was successfully treated with clipping and angioplasty. We also performed whole-genome sequencing to identify potential pathogenic gene polymorphisms.

CASE PRESENTATION

Chief complaints

A 3-year-old boy presented with intermittent non-projectile vomiting after a brief episode of syncope.

History of present illness

There was no clear triggering events for the emergence of symptoms. There was no blood in the gastric contents. Upon admission, the boy was lethargic but able to respond to command.

History of past illness

He had no history of trauma or surgery and no family history of cardiovascular diseases.

Physical examination

The Glasgow Coma Scale score was 14. Hunt-Hess grade was III. Pupil reflex was normal. The muscle strength was grade III in the left leg.

Laboratory examinations

With the exception of increased white blood cell count (8.58 × 10⁹/L), the laboratory test results were normal.

Imaging examinations

Computed tomography (CT) scan showed subarachnoid hemorrhage in the lateral fissure cistern and a small amount of blood in the right lateral ventricle (Figure 1A). CT angiography showed ruptured aneurysm in the right middle cerebral artery (Figure 1B-D). The intracranial aneurysm (IA) was 16 mm × 14 mm, with a wide neck. The pearl-and-string sign (proximal stenosis and distal stenosis in the intracranial aneurysm) was consistent with dissecting aneurysm (Figure 1C and D), as previously reported[15].

Figure 1 Preoperative imaging examination. A: Subarachnoid hemorrhage caused by ruptured intracranial dissecting aneurysm (IDA); B: Computed tomography angiography shows intracranial aneurysm in the right medical council on alcohol; C and D: Pearl-and-string sign of IDAs (focal stenoses proximally and distally, which are noted by white arrows.

FINAL DIAGNOSIS

Based on these features, a diagnosis of IDA was established.

TREATMENT

Surgery was conducted using a pterional approach under general anesthesia. After adequate exposure of the parent artery, an IA was apparent at the junction between M1 and M2. There was severe stenosis in the proximal part of the aneurysm. The aneurysm wall was extremely thin. The normal anatomical structure of the parent artery has been apparently destroyed. The aneurysm was opened, and the blood clot within the aneurysm and the patent artery was removed. Then the IA was clipped (Figure 2A and B). The normal anatomical structure of the parent artery was restored and the parent vessel remained patent. IDA lesion was resected and tissue specimen was sent to pathologic examination (Figure 2B and C).

Figure 2 Clipping and angioplasty for intracranial dissecting aneurysms, and pathological examination. A: The aneurysm was clipped; B: The wall of the intracranial dissecting aneurysm was very thin, and a thrombus was adhered to the wall; C: The intracranial dissecting aneurysm was resected and sent for pathological examination. Pathological examination indicated irregular and malformed vascular wall structure with inflammatory infiltration.

OUTCOME AND FOLLOW-UP

CT angiography was conducted 2 wk later, and showed no aneurysm; the parent artery was patent (Figure 3A-C). Neurologic symptoms and signs gradually improved. At the 1 mo follow-up visit, the boy was healthy, with the exception of slight muscle weakness in the left leg (grade IV). At 3 years later, the patient had completely recovered. CT angiography revealed normal blood supply to the brain (Figure 3D).

Figure 3 Postoperative computed tomography angiography examination and follow-up. A-C: Postoperative computed tomography angiography examination indicated that the aneurysm had been resected, and the blood flow of the constructed medical council on alcohol was unobstructed; D: The 3-year follow-up showed no recurrence.

Pathogenic variants

Whole-genome sequencing (Novogene, Beijing, China) of the blood sample followed by bioinformatics analysis according to the American College of Medical Genetics guidelines[16] revealed 13 candidate genes (Table 1). Next, we searched the PubMed database using the keyword “intracranial aneurysm” or “dissecting,” and “genes including pathogenic variation.” The literature review suggested an association between the rs78977446 variant of the ADAMTS13 gene and pediatric IDA. Briefly, ADAMTS13 participates in the inflammatory processes and vascular remodeling in IA[17,18]. Genetic variants, transcription abnormality, and methylation changes in the ADAMTS genes may be an important factor for IA[19]. In addition to IA, an autopsy study of 31 cases of aortic dissections revealed much higher frequency (0.1613) of the rs11575933 variant of the ADAMTS13 gene in aortic dissections[20] vs healthy control subjects (https://www.ncbi.nlm.nih.gov/snp/?term=rs11575933).

Table 1 Pathogenic variants found by American College of Medical Genetics guidelines.

Chromosome	Position	Variation	REF	ALT	Function	Gene	SIFT	Mutation taster	CADD
1	47610522	rs570554271	C	T	Stopgain	CYP4A22	-	1, A	10.070978, 36
2	234637905	rs45625338	C	T	Missense	UGT1A3	0.0, D	1, D	2.458692, 19.20
8	145699712	-	G	A	Missense	FOXH1	0.0, D	1, D	6.334943, 29.3
9	136310917	rs78977446	C	T	Missense	ADAMTS13	0.081, T	1, N	0.962795, 10.45
11	17482222	rs185040406	C	T	Missense	ABCC8	0.07, T	0.777604, N	3.415216, 23.0
12	85266484	rs12424429	G	A	Missense	SLC6A15	0.295, T	0.975276, N	-
13	100518634	rs41281112	C	T	Stopgain	CLYBL	-	1, A	8.514350, 35
14	75514138	rs28756990	C	A	Missense	MLH3	0.034, D	1, N	2.798595, 21.4
16	3705465	rs77254040	C	G	Missense	DNASE1	0.007, D	1, D	3.289682, 22.8
18	29867688	rs3744921	T	C	Missense	GAREM1	0.22, T	0.999954, D	1.071666, 11.06
19	4157148	rs77002741	G	A	Missense	CREB3L3	0.169, T	1, N	1.858481, 15.34
19	39898667	rs3746083	C	T	Synonymous	ZFP36	-	-	-
22	50523267	rs184241759	C	T	Missense	MLC1	0.007, D	1, N	3.434483, 23.0

CADD score > 15 means that the variation affects protein function. ALT: Mutation-type; REF: Reference. A SIFT score indicates whether the variation is likely to cause changes in protein structure or function: D: Deleterious (sift ≤ 0.05); T: Tolerated (sift > 0.05). MutationTaster represents the effect of the mutation on the protein sequence: A: Disease_causing_automatic; D: Disease_causing; N: Polymorphism; P: Polymorphism_automatic.

DISCUSSION

Treatment of ruptured IDA

IDA can be classified into two types. In type 1 IDA, the dissection is located between the elastic layer and media layer, and causes ischemic stroke. In type 2 IDA, the dissection occurs between the media and adventitia, and causes SAH[21].

Treatment options for type 2 IDA include microsurgical clipping, coiling embolization, triple stent, trapping[22], bypass[23], wrapping, and complete exclusion[24]. The choice of these treatment modalities remains controversial[25].

As an endovascular interventional therapy, clipping has been frequently used in pediatric IDA of the posterior circulation[26-28]. It does not require craniotomy and thus is associated with minimal surgical trauma. The IDA in the index case was relatively large, and was ruptured. Thus, controlling bleeding and preventing rebleeding were the primary aims of the treatment[29]. For this rare ruptured large dissecting aneurysm, microsurgery clipping and patent vessel remodeling may have a lower probability of long-term recurrence. More importantly, the lesions can be visualized during the microsurgery. Blood clot in the parent artery was cleared to establish the normal anatomy of the parent artery. IDA, which is similar to the saccular aneurysm in the same location, has the risk of rebleeding during the acute stage[30]. Also, recurrence after several years has been reported[31]. As a result, long-term monitoring is required.

Genetic indications and precision medication

Sequencing analysis followed by bioinformatics analysis and literature review suggested that the rs78977446 variant of the ADAMTS13 gene is a risk for pediatric IDA. IDA is more common in children than in adults, indicating a genetic contribution, but genetic studies for pediatric IDA are rare. In a previous study, the mutational rate was significantly higher in intracranial vertebral–basilar artery dissection cases than in controls[32]. RNF213 rs112735431 (c.14576G>A) frequency is significantly lower in patients with intracranial vertebral artery dissection. The genetic predisposition to IDA in the index case may form the basis of future recurrence, and physicians should be aware of the unique circumstance of each patient[33].

CONCLUSION

In summary, clipping and angioplasty are appropriate treatments for ruptured IDA in the anterior cerebral circulation. The rs78977446 variant of the ADAMTS13 gene is a risk factor for pediatric IDA.