Basic Study
Copyright ©The Author(s) 2018.
World J Clin Cases. Aug 16, 2018; 6(8): 183-191
Published online Aug 16, 2018. doi: 10.12998/wjcc.v6.i8.183
Figure 1
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Figure 1 Inhibition of hepatitis B virus deoxyribonucleic acid and hepatitis B surface antigen by anti-gene-locked nucleic acid, anti-sense-locked nucleic acid, and lamivudine. A: The inhibition rate of HBsAg; B: The inhibition rate of hepatitis B virus (HBV) deoxyribonucleic acid. aSignificant (P < 0.05) HBsAg and HBV DNA expression alterations between lamivudine, anti-sense-LNA vs anti-gene-LNA. Values are mean ± SD. LNA: Locked nucleic acid; HBsAg: HBV surface antigen.
Figure 2
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Figure 2 Hepatitis B virus S-mRNA expression levels in the liver of transgenic mice (mean ± SD, n = 7). A: Electrophoregram showing HBV S and GAPDH bands; B: Relative expression levels of HBV S-mRNA. aSignificant (P < 0.05) S-mRNA expression alterations between blank (1), unrelated sequence (2), lamivudine (3) vs Anti-sense-LNA (4) and Anti-gene-LNA (5); cSignificant (P < 0.05) S-mRNA expression alterations between Anti-sense-LNA (4) vs Anti-gene-LNA (5). Values are mean ± SD. M: DNA marker; LNA: Locked nucleic acid; HBV: Hepatitis B virus.
Figure 3
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Figure 3 Immunohistochemical detection (× 200) of hepatitis B surface antigen positive cells in liver tissues of transgenic mice. aSignificant (P < 0.05) HBsAg positive cells expression alterations between blank (A),unrelated sequence (B), lamivudine (C) vs anti-sense-LNA (D) and anti-gene-LNA (E); cSignificant (P < 0.05) HBsAg positive cells expression alterations between anti-sense-LNA (D) vs anti-gene-LNA (E). Values are mean ± SD; F: Positive HBsAg cells. LNA: Locked nucleic acid; HBV: Hepatitis B virus; HBsAg: HBV surface antigen.
Figure 4
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Figure 4 Morphological changes in liver sections obtained from transgenic mice infected with hepatitis B virus. Mice were sacrificed, and the livers were harvested and prepared for observation on the 7th d after treatment. A: Blank control group; B: Unrelated sequence control group; C: Lamivudine control group; D: Anti-sense-LNA treatment control group; E: Anti-gene-LNA treatment group. Liver sections were stained with hematoxylin and eosin (original magnification, × 200). There were no significant morphological changes in the liver among groups. LNA: Locked nucleic acid.
Figure 5
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Figure 5 Morphological changes in kidney sections obtained from transgenic mice infected with hepatitis B virus. Mice were sacrificed, and the kidneys were harvested and prepared for observation on the 7th d after treatment. A: Blank control group; B: Unrelated sequence control group; C: Lamivudine control group; D: Anti-sense-LNA treatment control group; E: Anti-gene-LNA treatment group. Kidney sections were stained with hematoxylin and eosin (original magnification, × 200). There were no significant morphological changes in the kidney among groups. LNA: Locked nucleic acid.