Correlations between microbial communities in stool and clinical indicators in patients with metabolic syndrome

Figure 1 Relative abundance of top 10 species at phylum and genus level between two groups. A: Relative abundance of top 10 species at phylum level between two groups; B: Relative abundance of top 10 species at genus level between two groups. Each column represents one group and different colors indicate different phylum or genus in the microbiota composition. The top 10 were listed. Relative abundance: the average of sample abundances in a group.

Figure 2 Species classification tree between two groups. Different colors in circles indicated different groups, the size of the sector indicated the relative abundance of the group in that category.

Figure 3 Abundance of top 10 species at genus level in two groups respectively. Each column represents one sample and different colors indicate different genus in the microbiota composition. The top 10 genus were listed.

Figure 4 Rarefaction curve and rank abundance curve of two groups. A: Rarefaction curve of two groups. The abscissa was the number of sequencing samples taken at random, the ordinate was the number of species corresponding to the number of samples sequenced. The different samples were marked with different colors; B: Rank abundance curve of two groups. The abscissa was the ordinal number sorted by the abundance of operational taxonomic units, the ordinate was the relative abundance of the operational taxonomic units in the corresponding sample. The different samples were marked with different colors.

Figure 5 Box diagram of ACE, Simpson and Shannon index of two groups. Green and yellow boxplots denoted the relative abundance or diversity of samples in two groups respectively. The t-test was used to analyze the significant difference between the indices of different groups.

Figure 6 Clustering map of species abundance of two groups. The vertical direction was the sample information and the horizontal direction was the species annotation information. The corresponding valued of the heat map is the log 2 converted relative abundance of each line of species.

Figure 7 Principal component analysis results of two groups. The abscissa indicated the first principal component, the percentage indicated the contribution of the first principal component to the sample difference; the ordinate indicated the second principal component, and the percentage indicated the contribution of the second principal component to the sample difference; each point in the figure represented a sample, and the same group of samples used the same color. PCA: Principal component analysis.