Published online Apr 16, 2018. doi: 10.12998/wjcc.v6.i4.54
Peer-review started: January 2, 2018
First decision: January 18, 2018
Revised: February 2, 2018
Accepted: March 7, 2018
Article in press: March 7, 2018
Published online: April 16, 2018
To analyze the bacterial community structure and distribution of intestinal microflora in people with and without metabolic syndrome and combined these data with clinical indicators to determine relationships between selected bacteria and metabolic diseases.
Faecal samples were collected from 20 patients with metabolic syndrome and 16 controls at Cangnan People’s Hospital, Zhejiang Province, China. DNA was extracted and the V3-V4 regions of the 16S rRNA genes were amplified for high throughput sequencing. Clear reads were clustered at the 97% sequence similarity level. α and β diversity were used to describe the bacterial community structure and distribution in patients. Combined with the clinical indicators, further analysis was performed.
Bacteroidetes, Firmicutes, Actinobacteria, Proteobacteria, Fusobacteria were the dominant phyla, and Prevotella, Bacteroides and Faecalibacterium was the top three genera in faecal samples. α diversity analysis showed that the species richness of metabolic syndrome samples (group D) was significantly higher than the control (group C) (P < 0.05), and the microbial diversity of group C was greater than that of group D. According to the principal co-ordinates analysis, the samples of group C clustered more tightly, indicating that the distribution of bacteria in healthy patients was similar. The correlation analysis showed that alkaline phosphatase was negatively correlated with the abundance of Prevotella (P < 0.05). There was a negative correlation between low-density lipoprotein and the abundance of Ruminococcus (P < 0.05) and a positive correlation between the high-density lipoprotein and the abundance of Ruminococcus (P < 0.05). The total protein and the alanine aminotransferase was positively correlated with the abundance of Peptostreptococcus (P < 0.05).
The changes microbial communities can be used as an indicator of metabolic syndrome, and Prevotella may be a target microorganism in patients with metabolic syndrome.
Core tip: We amplified the V3-V4 regions of the 16S rDNA from faecal samples to analysis the bacterial community structure and distribution of intestinal microflora in patients with metabolic syndrome combined with clinical indicators. The results showed that the species of metabolic syndrome patients was significantly higher than that of controls, and the microbial diversity of controls was greater than that of metabolic syndrome patients. The correlation analysis showed that alkaline phosphatase was negatively correlated with the abundance of Prevotella, indicating that Prevotella may be a target microorganism in patients with metabolic syndrome.