Prognostic role of multiple abnormal genes in non-small-cell lung cancer

Figure 1 Volcano map of gene expression data of 533 cancer tissues and 53 normal tissues from The Cancer Genome Atlas database reveals a large number of upregulated (red) and downregulated (green) genes. LUAD: Lung adenocarcinoma.

Figure 2 Two-dimensional heat map of various parameters plotted to intuitively observe patients' basic indicators and gene expression. OS: Overall survival.

Figure 3 Bubble chart and box chart show that RET, KIT, and TERT exhibit significantly different expression levels between the two groups. The samples were grouped based on the epidermal growth factor receptor and KRAS mutation status with more than 20 samples in each group.

Figure 4 Analysis of survival of patients with single-gene mutations. To amplify the single-gene effect, we considered genes with a Z score greater than 1 to be highly expressed and those with a Z score less than -1 to have a low expression level.

Figure 5  We used the Z score of 0 as the critical value and divided the four genes into two groups in which all had a high expression or a low expression level at the same time.

Figure 6 Heat map revealing data regarding gene mutations and basic clinical information collected from patients. LUAD: Lung adenocarcinoma; WT: Wild type; Del 19: Deletion of exon 19; LUSC: Lung squamous cell carcinoma; TKI: Tyrosine kinase inhibitor; OS: Overall survival.

Figure 7  Multivariate Cox regression analysis of impact of basic indicators and gene mutations.

Figure 8 Cox regression analysis revealed a significant effect of all gene mutations except for BRAF (P = 0. 02).

Figure 9 According to the number of mutations in the four tumor suppressor genes (TP53, PTEN, Rb1, and STK11), we classified those with more than one mutation into the greater than 1 group. gt1: Greater than 1; TKI: Tyrosine kinase inhibitor; OS: Overall survival.