Diagnosis of cytomegalovirus encephalitis using metagenomic next-generation sequencing of blood and cerebrospinal fluid: A case report

Figure 1 Timeline information of this case. NICU: Neonatal intensive care unit; mNGS: Metagenomic next-generation sequencing; CSF: Cerebrospinal fluid; CMV: Cytomegalovirus; HHV-6B: Human herpes virus 6B.

Figure 2 Electroencephalogram and magnetic resonance imaging. A: Video-electroencephalography during epileptic seizure on November 11, 2020; B: Ambulatory electroencephalogram during the remission period on November 11, 2020; C: Head magnetic resonance imaging examination on November 8, 2020; D: Head magnetic resonance imaging reexamination on November 18, 2020.

Figure 3 Next-generation sequencing and PCR analysis images. A: Blood metagenomic next-generation sequencing of the patient revealed a total of 614 specific reads of cytomegalovirus were detected, accounting for 23.93%; B: Cerebrospinal fluid metagenomic next-generation sequencing of the patient revealed 1 specific read of cytomegalovirus, accounting for 0.01%; C: Quantitative PCR detection showed that cytomegalovirus was positive in the blood on December 16, 2020. CSF: Cerebrospinal fluid.