Case Report
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Dec 6, 2019; 7(23): 4036-4043
Published online Dec 6, 2019. doi: 10.12998/wjcc.v7.i23.4036
In-vitro proliferation assay with recycled ascitic cancer cells in malignant pleural mesothelioma: A case report
Takashi Anayama, Mai Taguchi, Takehiro Tatenuma, Hironobu Okada, Ryohei Miyazaki, Kentaro Hirohashi, Motohiko Kume, Keisuke Matsusaki, Kazumasa Orihashi
Takashi Anayama, Mai Taguchi, Takehiro Tatenuma, Hironobu Okada, Ryohei Miyazaki, Kentaro Hirohashi, Motohiko Kume, Kazumasa Orihashi, Department of Surgery II, Kochi Medical School, Kochi University, Kochi 783-8505, Japan
Keisuke Matsusaki, Japanese CART Study Group, Kaname Second Clinic, Kanamecho Hospital, Tokyo 171-0043, Japan
Author contributions: Anayama T organized the entire study protocol and mainly wrote the manuscript; Taguchi M and Tatenuma T performed the CD-DST; Okada H and Kume M performed the KM-CART; Miyazaki R, Hirohashi K, and Kume M were responsible for the clinical treatment of the patient; Orihashi K was responsible for the entire study.
Informed consent statement: The patient agreed to all treatments. Every treatment was performed after obtaining written informed consent from the patient.
Conflict-of-interest statement: The authors have no conflicts of interest.
CARE Checklist (2016) statement: The authors have read the manuscript was prepared and revised according to the CARE Checklist (2016).
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Takashi Anayama, MD, PhD, Associate Professor, Department of Surgery II, Kochi Medical School, Kochi University, 2-5-1akebono-cho, Kochi-shi, Kochi 783-8505, Japan. anayamat@kochi-u.ac.jp
Telephone: +81-88-8802375 Fax: +81-88-8802376
Received: August 13, 2019
Peer-review started: August 13, 2019
First decision: September 23, 2019
Revised: November 8, 2019
Accepted: November 14, 2019
Article in press: November 14, 2019
Published online: December 6, 2019
Processing time: 114 Days and 22 Hours
Core Tip

Core tip: Massive ascites due to recurrent malignant mesothelioma was controlled with innovative cell-free and concentrated ascites reinfusion therapy, and the derived tumor cells were utilized secondarily in an in vitro cell growth assay that contributed to the personalized chemotherapy for the patient.