Case Report
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Aug 6, 2024; 12(22): 5208-5216
Published online Aug 6, 2024. doi: 10.12998/wjcc.v12.i22.5208
Diagnosis and treatment of refractory infectious diseases using nanopore sequencing technology: Three case reports
Qing-Mei Deng, Jian Zhang, Yi-Yong Zhang, Min Jia, Du-Shan Ding, Yu-Qin Fang, Hong-Zhi Wang, Hong-Cang Gu
Qing-Mei Deng, Hong-Zhi Wang, Science Island Branch, Graduate School of University of Science and Technology of China, Hefei 230031, Anhui Province, China
Qing-Mei Deng, Min Jia, Hong-Cang Gu, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Science, Hefei 230031, Anhui Province, China
Qing-Mei Deng, Jian Zhang, Yi-Yong Zhang, Min Jia, Du-Shan Ding, Yu-Qin Fang, Hong-Cang Gu, Medical Pathology Center, Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei 230031, Anhui Province, China
Co-corresponding authors: Hong-Zhi Wang and Hong-Cang Gu.
Author contributions: Deng QM, Zhang J and Jia M contributed to manuscript writing and editing; Ding DS and Fang YQ contributed to data collection; Zhang YY contributed to data analysis; Wang HZ and Gu HC contributed to conceptualization and supervision for co-corresponding authors; all authors have read and approved the final manuscript.
Supported by Research and Development Funding for Medical and Health Institutions, No. 2021YL007.
Informed consent statement: All patients gave informed consent.
Conflict-of-interest statement: The authors declare that they have no conflicts of interest to disclose.
CARE Checklist (2016) statement: The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hong-Zhi Wang, Doctor, Professor, Science Island Branch, Graduate School of University of Science and Technology of China, Yangqiao Road, Shushan District, Hefei 230031, Anhui Province, China. wanghz@hfcas.ac.cn
Received: April 10, 2024
Revised: May 29, 2024
Accepted: June 17, 2024
Published online: August 6, 2024
Processing time: 82 Days and 23.3 Hours
Core Tip

Core Tip: Routine culture methods have traditionally been the primary clinical approach for detecting pathogenic microorganisms. However, this article reports on three cases of refractory infectious diseases caused by pathogens that are not easily detected through routine culture methods or are susceptible to inhibition by the growth of other normal flora during the culture process. Nanopore sequencing technology offers a novel approach to identifying the source of infection, achieving precise and prompt pathogen detection, and guiding the use of clinical antimicrobial drugs.