Observational Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Feb 26, 2023; 11(6): 1318-1329
Published online Feb 26, 2023. doi: 10.12998/wjcc.v11.i6.1318
Metformin effect on internal carotid artery blood flow assessed by area under the curve of carotid artery Doppler in women with polycystic ovarian syndrome
Wisam Akram, Wassan Nori, Muna Abdul Ghani Zghair
Wisam Akram, Wassan Nori, Department of Obstetrics and Gynecology, Mustansiriyah, Al Saydihya 10052, Baghdad, Iraq
Muna Abdul Ghani Zghair, Department of Radiology, Mustansiriyah, Al Saydihya 10052, Baghdad, Iraq
Author contributions: Akram W and Nori W designed research and reviewed the data, wrote and revised the letter; Zghair MAG collected and analyzed the data; All authors have read and agreed on the final version of the manuscript.
Institutional review board statement: The ethical committee of Mustansiriyah University approved the study dated February/21/2019 (IRB No. 115).
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: All authors declare that they have no potential or real conflicts related to this paper.
Data sharing statement: All data are available on reasonable request from the corresponding author.
STROBE statement: The authors have read the STROBE Statement—checklist of items, and the manuscript was prepared and revised according to the STROBE Statement—checklist of items.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Wassan Nori, PhD, Academic Editor, Academic Research, Senior Researcher, Department of Obstetrics and Gynecology, Mustansiriyah, No. 38, Al Amin Street, Al Saydihya 10052, Baghdad, Iraq. dr.wassan76@uomustansiriyah.edu.iq
Received: November 30, 2022
Peer-review started: November 30, 2022
First decision: January 19, 2023
Revised: January 19, 2023
Accepted: January 31, 2023
Article in press: January 31, 2023
Published online: February 26, 2023
Processing time: 85 Days and 12 Hours
ARTICLE HIGHLIGHTS
Research background

Insulin resistance (IR) is implicated in many aspects of polycystic ovarian syndrome (PCOS) pathogenesis. Metformin effectively decreased IR. Improved IR was evaluated via homeostatic model assessment for IR (HOMA-IR) and Doppler parameters; mainly carotid artery intima-media thickness (ICA-IMT). The area under the curve of the internal carotid artery (AUC-ICA) Doppler wave was examined as a helpful marker for determining IR among PCOS cases presented with menstrual irregularity and treated by metformin over 6 mo.

Research motivation

Much research has shown that surrogate measures of insulin sensitivity are no longer sufficient for evaluating insulin sensitivity, which has increased the need for new direct methods. Demonstrating changes in blood flow mirrored by AUC appeared to be more dependable; as indicated in earlier work, ICA-IMT was already examined in PCOS.

Research objectives

To ascertain if IR is related to the AUC-ICA Doppler in PCOS-affected women with menstrual irregularities. The second goal is to analyze the reliability of AUC-ICA as a helpful marker for monitoring IR in women who have received metformin treatment.

Research methods

The study enrolled 54 PCOS women in a cross-sectional study. Anthropometric data included patient age, body mass index (BMI), menstrual cycle days, biochemical parameters: serum cholesterol, low and high-density lipoprotein, sex hormone-binding globulin, fasting blood glucose, and HOMA-IR, hormonal parameters: testosterone, luteinizing hormone over follicle-stimulating hormone ratio, and ultrasonic parameters: (CIMT, PI, RI, and AUC-ICA). Measurements of the systolic and diastolic wave height were repeated in order to evaluate the AUC-ICA following metformin tab-500 mg; three times/d for 6 mo. Metformin caused a progressive reduction in BMI, menstrual cycle days, biochemical hormonal, and Doppler parameters (CIMT, PI, RI, and AUC-ICA). AUC-ICA correlated strongly to all PCOS parameters. AUC-ICA correlated inversely with treatment time (r = -0.98, P < 0.001) and positively with HOMA-IR (r = 0.98, P < 0.0001). Via the best subset regression model, the AUC-ICA had the highest predictive value for HOMA-IR.

Research results

BMI, menstrual cycle days, biochemical hormonal, and Doppler markers (CIMT, PI, RI, and AUC-ICA were all gradually reduced by metformin treatment). All PCOS indicators and AUC-ICA had significant correlations. AUC-ICA had a negative correlation (r = -0.98, P < 0.001) with treatment time and a positive correlation (r = 0.98, P0.0001) with HOMA-IR. The AUC-ICA demonstrated the best subset regression model's maximum predictive value for HOMA-IR.

Research conclusions

AUC-ICA was a reliable marker for the assessment of IR, especially during metformin medication. AUC-ICA preceded PI, RI, and CIMT and showed a high, meaningful correlation to other PCOS markers. For further use in practice, more research is suggested.

Research perspectives

The area under the curve of the internal carotid artery had a significant correlation with HOMA-IR and the length of metformin therapy, not to mention it has a superior cost-benefit analysis over HOMA-IR. AUC-ICA is a reliable indicator of IR, follow-up, and prognostic value, particularly while using metformin.