Published online Jan 16, 2017. doi: 10.12998/wjcc.v5.i1.1
Peer-review started: June 27, 2016
First decision: August 11, 2016
Revised: August 27, 2016
Accepted: October 25, 2016
Article in press: October 27, 2016
Published online: January 16, 2017
To investigated the association between the tumor cells’ expression of E-cadherin and the numbers of several types of inflammatory cells infiltrating into the invasive portion of gallbladder cancer (GBC).
We analyzed 50 GBC cases for which a sufficient amount of tumor tissues for tissue microarray (TMA) had been saved. Three tissue cores (3.0 mm) of invasive lesion from each case were used for the TMA. The 4-μm cut sections on slides were immunostained using primary antibodies including E-cadherin for cancer cells, leukocyte common antigen for leukocyte, myeloperoxidase for neutrophils, CD3 for T cells, CD4 for helper T cells, CD8 for killer T cells, CD20 for B cells and CD68 for macrophages. The immunostained slides were digitally analyzed by imaging analysis software.
A significant inverse correlation between the number of infiltrating CD8+ cells at invasive areas and the expression of E-cadherin by cancer cells was observed (P = 0.0001), although the degree of this correlation was relatively weak (R = 0.32). The number of CD8+ cells and the cancer cells’ E-cadherin expression were also significantly correlated with tumor differentiation (well-differentiated vs poorly differentiated) (P = 0.0467 and P = 0.0294, respectively). Inverse correlation of T-stage and the number of CD8+ cell inﬁltration was observed with statistical significance in comparison of T2 and T3 cases (P = 0.0324).
Our findings indicate an inverse correlation of CD8+ T cell infiltration and cancer cells’ E-cadherin expression at invasive areas of GBC. Further analyses are essential to test these findings.
Core tip: We analyzed the association between the expression of E-cadherin in tumor cells and the type and amount of inflammatory cells infiltrating into the invasive portion of gallbladder cancer, using tissue microarray and imaging analyses. The results indicated an inverse correlation of CD8+ T cell infiltration and E-cadherin expression by cancer cells at invasive areas of gallbladder cancer. Further analyses are essential to test these results.