Flores IL, Santos-Silva AR, Coletta RD, Leme AFP, Lopes MA. Low expression of angiotensinogen and dipeptidyl peptidase 1 in saliva of patients with proliferative verrucous leukoplakia. World J Clin Cases 2016; 4(11): 356-363 [PMID: 27900324 DOI: 10.12998/wjcc.v4.i11.356]
Corresponding Author of This Article
Marcio Ajudarte Lopes, DDS, PhD, Oral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Av. Limeira, 901, Bairro Areão, Piracicaba, Sao Paulo 13414-903, Brazil. malopes@fop.unicamp.br
Research Domain of This Article
Dentistry, Oral Surgery & Medicine
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Clin Cases. Nov 16, 2016; 4(11): 356-363 Published online Nov 16, 2016. doi: 10.12998/wjcc.v4.i11.356
Low expression of angiotensinogen and dipeptidyl peptidase 1 in saliva of patients with proliferative verrucous leukoplakia
Isadora Luana Flores, Alan Roger Santos-Silva, Ricardo Della Coletta, Adriana Franco Paes Leme, Marcio Ajudarte Lopes
Isadora Luana Flores, Alan Roger Santos-Silva, Ricardo Della Coletta, Marcio Ajudarte Lopes, Oral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo 13414-903, Brazil
Adriana Franco Paes Leme, Mass Spectrometry Laboratory, Brazilian Biosciences National Laboratory (LNBio), Campinas, São Paulo 13083-970, Brazil
Author contributions: All of the authors contributed to the manuscript.
Institutional review board statement: This study followed the guidelines of the Declaration of Helsinki and Tokyo for research in humans, and have been approved by the local ethics board of the University of Campinas (protocol number: 105/2013).
Informed consent statement: All patients received verbal explanations concerning the study before signing a written informed consent.
Conflict-of-interest statement: None.
Data sharing statement: None.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Marcio Ajudarte Lopes, DDS, PhD, Oral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Av. Limeira, 901, Bairro Areão, Piracicaba, Sao Paulo 13414-903, Brazil. malopes@fop.unicamp.br
Telephone: +55-19-21065320 Fax: +55-19-21065218
Received: March 9, 2016 Peer-review started: March 10, 2016 First decision: May 19, 2016 Revised: August 24, 2016 Accepted: September 13, 2016 Article in press: September 18, 2016 Published online: November 16, 2016 Processing time: 249 Days and 19.1 Hours
Abstract
AIM
To elucidate the profile of the salivary proteome.
METHODS
Unstimulated whole mouth saliva was collected from 30 volunteers [15 proliferative verrucous leukoplakia (PVL) patients and 15 controls] and proteins were submitted for mass spectrometry-based proteomics using the discovery approach, followed by analyses of variance and logistic regression tests.
RESULTS
A total of two hundred and eighty-three proteins were confidently identified in saliva. By combining two low abundance proteins from the PVL group, angiotensinogen (AGT) and dipeptidyl peptidase 1 (DPP1), a model for group differentiation was built with a concordance index of 94.2%, identifying both proteins as potential etiologic biomarkers for PVL.
CONCLUSION
This study suggests that both AGT and DPP1 may be involved in developmental mechanisms of PVL.
Core tip: Saliva is a useful source for analysis of proteins that can be associated in many diseases, including proliferative verrucous leukoplakia (PVL). This study showed that angiotensinogen and dipeptidyl peptidase 1 may be important in PVL development.
