Retrospective Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Cases. Aug 16, 2024; 12(23): 5338-5345
Published online Aug 16, 2024. doi: 10.12998/wjcc.v12.i23.5338
Establishment and performance analysis of a new multiplex detection method for influenza an and B virus antigen
Cheng-Jing Xia, Bao-Hua Li, Yan-Ni Guo, Xiao-He Zhou, Run-Ling Zhang, Ying-No Niu
Cheng-Jing Xia, Bao-Hua Li, Yan-Ni Guo, Xiao-He Zhou, Run-Ling Zhang, Department of Clinical Laboratory, West Wing, Shenzhen Hospital (Guangming) of University of Chinese Academy of Sciences, Shenzhen 518106, Guangdong Province, China
Ying-No Niu, Laboratory, Nanjing Vazyme Biotech Co. Ltd, Nanjing 210033, Jiangsu Province, China
Author contributions: Xia CJ contributed to the manuscript writing, data collection and analysis; Xia CJ, Li BH, Guo YN, Zhou XH, Zhang RL and Niu YN collected data; Xia CJ and Niu YN were involved in the conceptualization and supervision of this manuscript; and all authors approved the final manuscript.
Supported by Shenzhen Guangming District Soft Science Research Project, No. 2021R01097.
Institutional review board statement: This study was approved by the Ethic Committee of West Wing, Shenzhen Hospital (Guangming) of University of Chinese Academy of Sciences.
Informed consent statement: Patients were not required to give informed consent to the study because the analysis used anonymous clinical data that were obtained after each patient agreed to treatment by written consent.
Conflict-of-interest statement: We have no financial relationships to disclose.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Cheng-Jing Xia, Doctor, Associate Chief Technician, Department of Clinical Laboratory, West Wing, Shenzhen Hospital (Guangming) of University of Chinese Academy of Sciences, No. 4253 Songbai Road, Matian Street, Guangming District, Shenzhen 518106, Guangdong Province, China. cjxia1976@163.com
Received: April 13, 2024
Revised: June 4, 2024
Accepted: June 20, 2024
Published online: August 16, 2024
Processing time: 82 Days and 22.4 Hours
Abstract
BACKGROUND

Influenza A and B virus detection is pivotal in epidemiological surveillance and disease management. Rapid and accurate diagnostic techniques are crucial for timely clinical intervention and outbreak prevention. Quantum dot-encoded microspheres have been widely used in immunodetection. The integration of quantum dot-encoded microspheres with flow cytometry is a well-established technique that enables rapid analysis. Thus, establishing a multiplex detection method for influenza A and B virus antigens based on flow cytometry quantum dot microspheres will help in disease diagnosis.

AIM

To establish a codetection method of influenza A and B virus antigens based on flow cytometry quantum dot-encoded microsphere technology, which forms the foundation for the assays of multiple respiratory virus biomarkers.

METHODS

Different quantum dot-encoded microspheres were used to couple the monoclonal antibodies against influenza A and B. The known influenza A and B antigens were detected both separately and simultaneously on a flow cytometer, and the detection conditions were optimized to establish the influenza A and B antigen codetection method, which was utilized for their detection in clinical samples. The results were compared with the fluorescence quantitative polymerase chain reaction (PCR) method to validate the clinical performance of this method.

RESULTS

The limits of detection of this method were 26.1 and 10.7 pg/mL for influenza A and B antigens, respectively, which both ranged from 15.6 to 250000 pg/mL. In the clinical sample evaluation, the proposed method well correlated with the fluorescent quantitative PCR method, with positive, negative, and overall compliance rates of 57.4%, 100%, and 71.6%, respectively.

CONCLUSION

A multiplex assay for quantitative detection of influenza A and B virus antigens has been established, which is characterized by high sensitivity, good specificity, and a wide detection range and is promising for clinical applications.

Keywords: Influenza A; Influenza B; Quantum dot microspheres; Antigen detection; Multiplex detection

Core Tip: Respiratory viruses primarily target and affect the respiratory system, such as the influenza A and B viruses, highly contagious and can spread through various means. The detection of influenza A and B virus antigens is significant for the diagnosis, treatment, and prevention of influenza. In this study, a multiplex detective method for influenza A and B virus antigens was developed using flow cytometry quantum dot microspheres. The multiplex assay is characterized by high sensitivity, good specificity, and a broad detection range, making it a promising tool for clinical applications.