Published online Aug 16, 2024. doi: 10.12998/wjcc.v12.i23.5338
Revised: June 4, 2024
Accepted: June 20, 2024
Published online: August 16, 2024
Processing time: 82 Days and 22.4 Hours
Influenza A and B virus detection is pivotal in epidemiological surveillance and disease management. Rapid and accurate diagnostic techniques are crucial for timely clinical intervention and outbreak prevention. Quantum dot-encoded microspheres have been widely used in immunodetection. The integration of quantum dot-encoded microspheres with flow cytometry is a well-established technique that enables rapid analysis. Thus, establishing a multiplex detection method for influenza A and B virus antigens based on flow cytometry quantum dot microspheres will help in disease diagnosis.
To establish a codetection method of influenza A and B virus antigens based on flow cytometry quantum dot-encoded microsphere technology, which forms the foundation for the assays of multiple respiratory virus biomarkers.
Different quantum dot-encoded microspheres were used to couple the monoclonal antibodies against influenza A and B. The known influenza A and B antigens were detected both separately and simultaneously on a flow cytometer, and the detection conditions were optimized to establish the influenza A and B antigen codetection method, which was utilized for their detection in clinical samples. The results were compared with the fluorescence quantitative poly
The limits of detection of this method were 26.1 and 10.7 pg/mL for influenza A and B antigens, respectively, which both ranged from 15.6 to 250000 pg/mL. In the clinical sample evaluation, the proposed method well correlated with the fluorescent quantitative PCR method, with positive, negative, and overall compliance rates of 57.4%, 100%, and 71.6%, respectively.
A multiplex assay for quantitative detection of influenza A and B virus antigens has been established, which is characterized by high sensitivity, good specificity, and a wide detection range and is promising for clinical applications.
Core Tip: Respiratory viruses primarily target and affect the respiratory system, such as the influenza A and B viruses, highly contagious and can spread through various means. The detection of influenza A and B virus antigens is significant for the diagnosis, treatment, and prevention of influenza. In this study, a multiplex detective method for influenza A and B virus antigens was developed using flow cytometry quantum dot microspheres. The multiplex assay is characterized by high sensitivity, good specificity, and a broad detection range, making it a promising tool for clinical applications.