Haplotype analysis of long-chain non-coding RNA NONHSAT102891 promoter polymorphisms and depression in Chinese individuals: A case-control association study

Table 1 Genetic model genotype and allele frequencies of two long-chain non-coding RNA NONHSAT102891 promoter polymorphisms among depressed patients and controls and association with risk of depression, n (%)

Models	Polymorphisms	Control (n = 329)	Patients (n = 480)	Adjusted OR (95%CI)	P value
	rs6230
Codominant	TT	109 (33.1)	170 (35.4)	1
	TC	173 (52.6)	250 (52.1)	0.91 (0.66-1.24)	0.53
	CC	47 (14.3)	60 (12.5)	0.82 (0.52-1.30)	0.41
Dominant	TT	109 (33.1)	170 (35.4)	1
	TC/TT	220 (66.9)	310 (64.6)	0.79 (0.57-1.10)	0.17
Recessive	TT/TC	282 (85.7)	420 (87.5)	1
	CC	47 (14.3)	60 (12.5)	0.77 (0.49-1.23)	0.28
Allele	T	391 (59.4)	590 (61.5)	1
	C	267 (40.6)	370 (38.5)	0.92 (0.75-1.12)	0.40
	rs3792747
Codominant	TT	208 (63.2)	326 (67.9)	1
	TC	113 (34.3)	137 (28.5)	0.77 (0.57-1.05)	0.17
	CC	8 (2.4)	17 (3.5)	1.36 (0.57-3.20)	0.48
Dominant	TT	208 (63.2)	326 (67.9)	1
	TC/CC	121 (36.8)	154 (32.1)	0.84 (0.60-1.17)	0.31
Recessive	TT/TC	321 (97.6)	463 (96.5)	1
	CC	8 (2.4)	17 (3.5)	1.47(0.63-3.45)	0.36
Allele	T	529 (80.4%)	789 (82.2)	1	0.65
	C	129 (19.6)	171 (17.8)	0.89 (0.69-1.15)	0.36

Odds ratio was adjusted by age and sex. OR: Odds ratio; CI: Confidence interval.

Table 2 Stratified analyses of the rs6230 and rs3792747 polymorphisms in depressed patients

Variables	Frequency (%)		Adjusted OR (95%CI)	P value
rs6230
Depressive episode	Severe	Mild
TT	100 (39.7)	70 (30.7)	1.00 (Ref)
TC	125 (49.6)	125 (54.8)	1.57 (1.05-2.36)	0.28
CC	27 (10.7)	33 (14.5)	1.69 (0.92-3.09)	0.088
TC/CC	152 (60.3)	158 (69.3)	1.59 (1.08-2.35)	0.019
Suicide attempt	Yes	No
TT	102 (34.9)	68 (36.2)	1.00 (Ref)
TC	154 (52.7)	96 (51.1)	1.06 (0.70-1.63)	0.78
CC	36 (12.3)	24 (12.8)	0.87 (0.45-1.68)	0.68
TC/CC	190 (65.1)	120 (63.8)	1.02 (0.68-1.54)	0.91
First-episode patient	Yes	No
TT	88 (35.5)	82 (35.3)	1.00 (Ref)
TC	131 (52.8)	119 (51.3)	1.07 (0.71-1.60)	0.75
CC	29 (11.7)	31 (13.4)	1.15 (0.63-2.10)	0.66
TC/CC	160 (64.5)	150 (64.7)	1.08 (0.73-1.58)	0.70
Family history	Yes	No
TT	37 (39.4)	133 (34.5)	1.00 (Ref)
TC	47 (50)	203 (52.6)	1.29 (0.79-2.11)	0.31
CC	10 (10.6)	50 (12.9)	1.44 (0.66-3.14)	0.35
TC/CC	57 (60.6)	253 (65.5)	1.30 (0.81-2.07)	0.28
rs3792747
Depressive episode	Severe	Mild
TT	176 (69.8)	150 (65.8)	1.00 (Ref)
TC/CC	76 (30.2)	78 (34.2)	1.21 (0.82-1.80)	0.33
Suicide attempt	Yes	No
TT	201 (68.8)	125 (66.5)	1.00 (Ref)
TC/CC	91 (31.2)	63 (33.5)	1.14 (0.75-1.72)	0.54
First-episode patient	Yes	No
TT	168 (67.7)	158 (68.1)	1.00 (Ref)
TC/CC	80 (32.3)	74 (31.9)	0.98 (0.66-1.44)	0.9
Family history	Yes	No
TT	64 (68.1)	262 (67.9)	1.00 (Ref)
TC/CC	30 (31.9)	124 (32.1)	1.00 (0.62-1.63)	1.00

Odds ratio was adjusted by age and gender. OR: Odds ratio; CI: Confidence interval.

Table 3 Haplotype analysis of rs3792747 and rs6230 in depressed patients and controls, n (%)

Haplotype	Depressed patients	Controls	OR (95%CI)	P value
T/A	433 (45.1)	284 (43.2)	1.00
T/G	356 (37.1)	245 (37.2)	0.95 (0.76-1.19)	0.67
C/A	157 (16.4)	107 (16.3)	0.96 (0.72-1.28)	0.79
C/G	14 (1.5)	22 (3.3)	0.42 (0.21-0.83)	0.01

OR: Odds ratio; CI: Confidence interval.

Table 4 Relative luciferase activity of control and case groups

Group	RFA	OR (95%CI)	P value
Control	1.58 ± 0.28	1.00
EV	1.52 ± 0.22	0.080 (-0.526-0.542)	0.973
p-G/C	1.59 ± 0.27	-0.006 (-0.540-0.528)	0.979
p-A/T	1.57 ± 0.29	0.060 (-0.474-0.594)	0.802

Data represent the mean ± SEM. RFA value: The ratio of luciferase activity of recombinant plasmid to that of Renilla. EV: Empty vector; OR: Odds ratio; CI: Confidence interval.