Case Control Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Psychiatry. Dec 19, 2023; 13(12): 1005-1015
Published online Dec 19, 2023. doi: 10.5498/wjp.v13.i12.1005
Haplotype analysis of long-chain non-coding RNA NONHSAT102891 promoter polymorphisms and depression in Chinese individuals: A case-control association study
Yue Li, Yi-Xi Wang, Xing-Ming Tang, Peng Liang, Jing-Jie Chen, Feng Jiang, Qiang Yang, Yun-Dan Liang
Yue Li, Yi-Xi Wang, Peng Liang, Jing-Jie Chen, Feng Jiang, Qiang Yang, Yun-Dan Liang, Department of Pathology and Pathophysiology, Chengdu Medical College, Chengdu 610500, Sichuan Province, China
Xing-Ming Tang, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
Author contributions: Liang YD designed the study and corrected the manuscript; Li Y performed the majority of experiments and wrote the manuscript; Wang YX, Tang XM, Liang P, Chen JJ, Jiang F, Yang Q participated to the data collection and analysis of human material, and commented on previous versions of the manuscript; all authors contributed to the study conception and design. All authors read and approved the final manuscript.
Supported by National Natural Science Foundation of China, No. 81901379; Chengdu Medical College Graduate Research Innovation Fund Project, No. YCX2023-01-03; National Undergraduate Training Program for Innovation and Entrepreneurship, No. 202113705034.
Institutional review board statement: The study was approved by the ethics committee of Chengdu medical college (Chengdu, China) (approval number: 201815).
Informed consent statement: All patients gave informed consent.
Conflict-of-interest statement: Dr. Liang reports grants from the National Natural Science Foundation of China, grants from the Chengdu Medical College Graduate Research Innovation Fund Project, grants from the National Undergraduate Training Program for Innovation and Entrepreneurship, during the conduct of the study.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at liangyundan2004@126.com
STROBE statement: The authors have read the STROBE Statement-checklist of items, and the manuscript was prepared and revised according to the STROBE Statement-checklist of items.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Yun-Dan Liang, PhD, Adjunct Associate Professor, Associate Professor, Department of Pathology and Pathophysiology, Chengdu Medical College, No. 783 Xindu Avenue, Chengdu 610500, Sichuan Province, China. liangyundan2004@126.com
Received: August 31, 2023
Peer-review started: August 31, 2023
First decision: September 14, 2023
Revised: October 13, 2023
Accepted: November 9, 2023
Article in press: November 9, 2023
Published online: December 19, 2023
Processing time: 110 Days and 5.1 Hours
ARTICLE HIGHLIGHTS
Research background

Depression is a common life-threatening and disabling mental illness, and long-chain non-coding RNA (lncRNA) abnormal expression may affect the pathophysiological processes of depression. Our previous study reported that the single-nucleotide polymorphism (SNP) rs155979 GC in the promoter region of lncRNA NONHSAT102891 affects depression susceptibility in a Chinese population.

Research motivation

The complex interplay of species between major depressive disorder and lncRNA remains unclear.

Research objectives

To explored associations between two SNPs and haplotypes within lncRNA NONHSAT102891 promoter region and depression susceptibility in Chinese population.

Research methods

We conducted a case-control study in a cohort of 480 patients with depression and 329 healthy controls, and performed genotyping by gene sequencing. The function of the two lncRNA NONHSAT102891 promoter G/C and A/T haplotypes was detected by dual-luciferase reporter assays of human embryonic kidney 293T transfected cells.

Research results

The degree of mild depressive episodes associated with the rs6230 TC/CC genotype increased by 1.59 times. The haploid analysis revealed linkage disequilibrium between rs3792747 and rs6230, and the double SNP CG haplotype was more common in the control group compared to case group, indicating that this haplotype significantly reduced the risk of depression (C/G vs T/A: odds ratio = 0.42, 95% confidence interval: 0.21-0.83, P = 0.01). There was no significant difference in the dual-luciferase reporter activity of the G/C and A/T haplotypes compared with the control group (P > 0.05).

Research conclusions

The rs3792747 and rs6230 CG haplotypes of the lncRNA NONHSA T102891 promoter may be associated with a reduced risk of depression in the Chinese population. However, further studies with a larger sample size are required to determine the reference expression range of biomarkers.

Research perspectives

This study provides insights into the early prediction and diagnosis of depression and important clues for development of tools that will facilitate more accurate diagnosis and treatment of depression in the clinic.